##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccI AflIII AscI AseI
AsnI AviII BcgI BciVI BclI
BglII BsaBI BsiWI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstZ17I
ClaI CpoI DrdI EcoRV EcoT22I
FbaI FseI FspI HpaI MamI
MfeI MluI MunI NcoI NheI
NotI NsiI PacI PaeR7I PmeI
PshAI PshBI PvuI RcaI RsrII
SalI SapI SbfI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3361 GGTACC G^GTACC
AccIII 2225 TCCGGA T^CCGGA
AclI 1576 AACGTT AA^CGTT
AflII 264 CTTAAG C^TTAAG
AgeI 1703 ACCGGT A^CCGGT
AhdI 3802 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 240 GTGCAC G^TGCAC
Aor51HI 4245 AGCGCT AGC^GCT
ApaLI 240 GTGCAC G^TGCAC
Asp718I 3361 GGTACC G^GTACC
AspEI 3802 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 13 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3345 GGATCC G^GATCC
BbrPI 4231 CACGTG CAC^GTG
BfrI 264 CTTAAG C^TTAAG
BsaAI 4231 CACGTG [CT]AC^GT[GA]
BsaI 4269 GAGACC GGTCTC(1/5)
BseAI 2225 TCCGGA T^CCGGA
BspEI 2225 TCCGGA T^CCGGA
BsrDI 4368 GCAATG GCAATG(2/0)
BstAPI 516 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3683 TTCGAA TT^CGAA
BstYI 3345 GGATCC [GA]^GATC[CT]
DraI 2720 TTTAAA TTT^AAA
DraIII 2263 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3802 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4245 AGCGCT AGC^GCT
KpnI 3361 GGTACC GGTAC^C
MflI 3345 GGATCC [GA]^GATC[CT]
MroI 2225 TCCGGA T^CCGGA
NdeI 2682 CATATG CA^TATG
NruI 2281 TCGCGA TCG^CGA
NspI 855 GCATGT [GA]CATG^[CT]
NspV 3683 TTCGAA TT^CGAA
PflFI 13 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1703 ACCGGT A^CCGGT
PmaCI 4231 CACGTG CAC^GTG
PmlI 4231 CACGTG CAC^GTG
Psp1406I 1576 AACGTT AA^CGTT
SacI 4026 GAGCTC GAGCT^C
ScaI 1307 AGTACT AGT^ACT
SexAI 2264 ACCTGGT A^CC[AT]GGT
SfiI 1209 GGCCCAGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3683 TTCGAA TT^CGAA
Tth111I 13 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2798 TCTAGA T^CTAGA
XhoII 3345 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 3346 GATCC GGATC(4/5)
3345 GGATC
AocI 2922 CCTAAGG CC^T[ACGT]AGG
3847 CCTCAGG
Asp700I 2802 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2913 GAACCACTTC
AvrII 22 CCTAGG C^CTAGG
170 CCTAGG
BlnI 22 CCTAGG C^CTAGG
170 CCTAGG
BlpI 705 GCTGAGC GC^T[ACGT]AGC
2160 GCTCAGC
Bpu1102I 705 GCTGAGC GC^T[ACGT]AGC
2160 GCTCAGC
BsaWI 1703 ACCGGT [AT]^CCGG[AT]
2225 TCCGGA
BsgI 514 GTGCAG GTGCAG(16/14)
1108 GTGCAG
BsiEI 2410 CGGCCG CG[GA][CT]^CG
3908 CGGCCG
BsmBI 1509 CGTCTC CGTCTC(1/5)
2184 CGTCTC
BsmI 2785 GAATGC GAATGC(1/-1)
2864 GAATGC
Bsp1407I 935 TGTACA T^GTACA
1622 TGTACA
BsrGI 935 TGTACA T^GTACA
1622 TGTACA
BstEII 1674 GGTCACC G^GT[ACGT]ACC
1791 GGTCACC
BstPI 1674 GGTCACC G^GT[ACGT]ACC
1791 GGTCACC
Bsu36I 2922 CCTAAGG CC^T[ACGT]AGG
3847 CCTCAGG
CelII 705 GCTGAGC GC^T[ACGT]AGC
2160 GCTCAGC
EagI 2410 CGGCCG C^GGCCG
3908 CGGCCG
EclXI 2410 CGGCCG C^GGCCG
3908 CGGCCG
Eco52I 2410 CGGCCG C^GGCCG
3908 CGGCCG
Eco81I 2922 CCTAAGG CC^T[ACGT]AGG
3847 CCTCAGG
EcoO65I 1674 GGTCACC G^GT[ACGT]ACC
1791 GGTCACC
EcoRI 94 GAATTC G^AATTC
656 GAATTC
MslI 1101 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1794 CACCTGGATG
NaeI 199 GCCGGC GCC^GGC
545 GCCGGC
NgoMIV 199 GCCGGC G^CCGGC
545 GCCGGC
SgrAI 198 CGCCGGCG C[GA]^CCGG[CT]G
544 CGCCGGCG
SmlI 264 CTTAAG C^T[CT][GA]AG
3494 CTTGAG
SspBI 935 TGTACA T^GTACA
1622 TGTACA
StuI 260 AGGCCT AGG^CCT
2565 AGGCCT
XmnI 2802 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2913 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmaCI XmaI