##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvaI AviII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsoBI BspDI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
CelII Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I FauI
FseI FspI HaeII Hin1I HpaI
KasI KpnI MaeII MamI MfeI
MluI MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 344 TCCGGA T^CCGGA
AhdI 1762 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3858 CCTAAGG CC^T[ACGT]AGG
AspEI 1762 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3037 CCTAGG C^CTAGG
BalI 3511 TGGCCA TGG^CCA
BamHI 1018 GGATCC G^GATCC
BanI 1969 GGTGCC G^G[CT][GA]CC
BanII 1605 GGGCTC G[GA]GC[CT]^C
BlnI 3037 CCTAGG C^CTAGG
BsaWI 344 TCCGGA [AT]^CCGG[AT]
BseAI 344 TCCGGA T^CCGGA
BsmBI 4693 GAGACG CGTCTC(1/5)
Bsp1407I 3629 TGTACA T^GTACA
BspEI 344 TCCGGA T^CCGGA
BsrGI 3629 TGTACA T^GTACA
Bst1107I 3917 GTATAC GTA^TAC
BstAPI 1538 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3003 CCAGTAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3917 GTATAC GTA^TAC
Bsu36I 3858 CCTAAGG CC^T[ACGT]AGG
DraIII 3676 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2164 GACTCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3511 TGGCCA [CT]^GGCC[GA]
Eam1105I 1762 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3858 CCTAAGG CC^T[ACGT]AGG
EcoNI 4594 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1626 ATGCAT ATGCA^T
HapII 345 CCGG C^CGG
HpaII 345 CCGG C^CGG
KspI 299 CCGCGG CCGC^GG
MluNI 3511 TGGCCA TGG^CCA
MroI 344 TCCGGA T^CCGGA
MscI 3511 TGGCCA TGG^CCA
MspI 345 CCGG C^CGG
NcoI 4280 CCATGG C^CATGG
NdeI 1247 CATATG CA^TATG
NsiI 1626 ATGCAT ATGCA^T
PflMI 4251 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4137 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 299 CCGCGG CCGC^GG
ScaI 4580 AGTACT AGT^ACT
SexAI 4127 ACCTGGT A^CC[AT]GGT
SspBI 3629 TGTACA T^GTACA
StuI 3804 AGGCCT AGG^CCT
Van91I 4251 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4494 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1766 ACATGT A^C[GA][CT]GT
3266 ACATGT
AlwNI 2224 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
4294 CAGGAGCTG
BbsI 2295 GTCTTC GAAGAC(2/6)
4342 GTCTTC
BclI 3651 TGATCA T^GATCA
3879 TGATCA
BmyI 1605 GGGCTC G[AGT]GC[ACT]^C
1970 GTGCCC
BpuAI 2295 GTCTTC GAAGAC(2/6)
4342 GTCTTC
BsmFI 1760 GGGAC GGGAC(10/14)
3144 GTCCC
BsmI 1624 GAATGC GAATGC(1/-1)
4182 GAATGC
Bsp1286I 1605 GGGCTC G[AGT]GC[ACT]^C
1970 GTGCCC
BspLU11I 1766 ACATGT A^CATGT
3266 ACATGT
BsrDI 4236 CATTGC GCAATG(2/0)
755 GCAATG
CfoI 130 GCGC GCG^C
1226 GCGC
DraII 3033 AGGGCCT [GA]G^G[ACGT]CC[CT]
3545 AGGGCCT
DsaI 299 CCGCGG C^C[GA][CT]GG
4280 CCATGG
EcoO109I 3033 AGGGCCT [GA]G^G[ACGT]CC[CT]
3545 AGGGCCT
EcoRV 2200 GATATC GAT^ATC
3583 GATATC
FbaI 3651 TGATCA T^GATCA
3879 TGATCA
HgaI 127 GACGC GACGC(5/10)
2959 GACGC
HhaI 130 GCGC GCG^C
1226 GCGC
HinP1I 130 GCGC G^CGC
1226 GCGC
HincII 2244 GTCAAC GT[CT]^[GA]AC
2813 GTTGAC
HindII 2244 GTCAAC GT[CT]^[GA]AC
2813 GTTGAC
HindIII 3326 AAGCTT A^AGCTT
3979 AAGCTT
MslI 543 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3621 CACCTTTGTG
NspI 1766 ACATGT [GA]CATG^[CT]
3266 ACATGT
SpeI 1107 ACTAGT A^CTAGT
2947 ACTAGT
XcmI 2430 CCACCTTTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3769 CCAGACTTAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApoI AseI
AsnI Asp700I AvaII BbvI BfaI
BfrI BglII BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI