##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AflII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbrPI BciVI
BclI BfrI BlnI BsaAI BsiWI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraIII Eam1105I Eco47III
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
MluI NcoI NdeI NotI NruI
NsiI NspV PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3409 GGTACC G^GTACC
AccI 2098 GTAGAC GT^[AC][GT]AC
AccIII 2024 TCCGGA T^CCGGA
AclI 772 AACGTT AA^CGTT
AflIII 2659 ACACGT A^C[GA][CT]GT
Alw44I 215 GTGCAC G^TGCAC
AocI 3566 CCTGAGG CC^T[ACGT]AGG
ApaLI 215 GTGCAC G^TGCAC
Asp718I 3409 GGTACC G^GTACC
BbvCI 3259 GCTGAGG CCTCAGC(-5/-2)
BglI 277 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4169 AGATCT A^GATCT
BseAI 2024 TCCGGA T^CCGGA
BsgI 132 CTGCAC GTGCAG(16/14)
BsmBI 3733 GAGACG CGTCTC(1/5)
BsmI 2538 GCATTC GAATGC(1/-1)
BspEI 2024 TCCGGA T^CCGGA
BstAPI 2899 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1956 GGTGACC G^GT[ACGT]ACC
BstPI 1956 GGTGACC G^GT[ACGT]ACC
Bsu36I 3566 CCTGAGG CC^T[ACGT]AGG
EagI 3794 CGGCCG C^GGCCG
EclXI 3794 CGGCCG C^GGCCG
Eco52I 3794 CGGCCG C^GGCCG
Eco81I 3566 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1956 GGTGACC G^GT[ACGT]ACC
HindIII 1097 AAGCTT A^AGCTT
KpnI 3409 GGTACC GGTAC^C
KspI 1448 CCGCGG CCGC^GG
MfeI 2793 CAATTG C^AATTG
MroI 2024 TCCGGA T^CCGGA
MunI 2793 CAATTG C^AATTG
NaeI 1698 GCCGGC GCC^GGC
NgoMIV 1698 GCCGGC G^CCGGC
NspI 1821 ACATGC [GA]CATG^[CT]
PacI 1017 TTAATTAA TTAAT^TAA
PflMI 154 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1493 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 772 AACGTT AA^CGTT
PstI 3182 CTGCAG CTGCA^G
PvuII 3513 CAGCTG CAG^CTG
SacII 1448 CCGCGG CCGC^GG
SfcI 3182 CTGCAG C^T[GA][CT]AG
SfiI 276 GGCCAGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SwaI 2281 ATTTAAAT ATTT^AAAT
Van91I 154 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Asp700I 1052 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3644 GAAAGTCTTC
BalI 539 TGGCCA TGG^CCA
4288 TGGCCA
BbeI 431 GGCGCC GGCGC^C
3490 GGCGCC
BcgI 134 GCACTCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
910 GCAGGGGCTTCG
BlpI 1000 GCTGAGC GC^T[ACGT]AGC
3515 GCTGAGC
Bpu1102I 1000 GCTGAGC GC^T[ACGT]AGC
3515 GCTGAGC
BsaWI 1773 TCCGGT [AT]^CCGG[AT]
2024 TCCGGA
Bsp1407I 2936 TGTACA T^GTACA
4031 TGTACA
BsrGI 2936 TGTACA T^GTACA
4031 TGTACA
BstYI 2519 GGATCT [GA]^GATC[CT]
4169 AGATCT
CelII 1000 GCTGAGC GC^T[ACGT]AGC
3515 GCTGAGC
DraI 2282 TTTAAA TTT^AAA
3988 TTTAAA
DrdI 1858 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4391 GACAGAGTTGTC
EarI 1139 GAAGAG CTCTTC(1/4)
3872 GAAGAG
KasI 431 GGCGCC G^GCGCC
3490 GGCGCC
Ksp632I 1139 GAAGAG CTCTTC(1/4)
3872 GAAGAG
MflI 2519 GGATCT [GA]^GATC[CT]
4169 AGATCT
MluNI 539 TGGCCA TGG^CCA
4288 TGGCCA
MscI 539 TGGCCA TGG^CCA
4288 TGGCCA
MslI 3219 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4370 CATGGCTGTG
NarI 431 GGCGCC GG^CGCC
3490 GGCGCC
NheI 258 GCTAGC G^CTAGC
746 GCTAGC
PaeR7I 455 CTCGAG C^TCGAG
3525 CTCGAG
SacI 458 GAGCTC GAGCT^C
3193 GAGCTC
SfoI 431 GGCGCC GGC^GCC
3490 GGCGCC
SspBI 2936 TGTACA T^GTACA
4031 TGTACA
StuI 2597 AGGCCT AGG^CCT
3613 AGGCCT
TfiI 3879 GAATC G^A[AT]TC
4025 GATTC
XcmI 1180 CCAACGATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3218 CCACCTGGGTGCTGG
XhoI 455 CTCGAG C^TCGAG
3525 CTCGAG
XhoII 2519 GGATCT [GA]^GATC[CT]
4169 AGATCT
XmnI 1052 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3644 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MamI MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
SspI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XmaCI XmaI