##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BbeI
BcgI BciVI BclI BfrI BglII
BsaHI BseAI BsiEI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AocI 1284 CCTCAGG CC^T[ACGT]AGG
AspI 623 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3397 CCTAGG C^CTAGG
BamHI 3675 GGATCC G^GATCC
BbrPI 277 CACGTG CAC^GTG
BbsI 2143 GAAGAC GAAGAC(2/6)
BlnI 3397 CCTAGG C^CTAGG
BlpI 3586 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3586 GCTCAGC GC^T[ACGT]AGC
BpuAI 2143 GAAGAC GAAGAC(2/6)
BsaAI 277 CACGTG [CT]AC^GT[GA]
BsaBI 2525 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2126 TCCGGT [AT]^CCGG[AT]
BspDI 1587 ATCGAT AT^CGAT
BspHI 961 TCATGA T^CATGA
BstAPI 3461 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 3675 GGATCC [GA]^GATC[CT]
Bsu36I 1284 CCTCAGG CC^T[ACGT]AGG
CelII 3586 GCTCAGC GC^T[ACGT]AGC
ClaI 1587 ATCGAT AT^CGAT
DrdI 3447 GACTCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1284 CCTCAGG CC^T[ACGT]AGG
EcoNI 2648 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2944 ATGCAT ATGCA^T
MamI 2525 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3675 GGATCC [GA]^GATC[CT]
NcoI 3072 CCATGG C^CATGG
NheI 2908 GCTAGC G^CTAGC
NsiI 2944 ATGCAT ATGCA^T
PaeR7I 831 CTCGAG C^TCGAG
PflFI 623 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 277 CACGTG CAC^GTG
PmlI 277 CACGTG CAC^GTG
RcaI 961 TCATGA T^CATGA
SacI 3729 GAGCTC GAGCT^C
SbfI 2788 CCTGCAGG CCTGCA^GG
SfiI 2768 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2788 CCTGCAGG CCTGCA^GG
Tth111I 623 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 831 CTCGAG C^TCGAG
XhoII 3675 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 295 GTAC GT^AC
1606 GTAC
AlwNI 2109 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2973 CAGAGCCTG
BglI 2246 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2769 GCCACCCTGGC
BsgI 59 CTGCAC GTGCAG(16/14)
650 GTGCAG
BsrBI 1122 CCGCTC CCGCTC(-3/-3)
2724 GAGCGG
BsrDI 1410 CATTGC GCAATG(2/0)
1107 GCAATG
BsrFI 3640 GCCGGC [GA]^CCGG[CT]
4034 GCCGGC
BsrI 644 ACTGG ACTGG(1/-1)
4202 ACTGG
Cfr10I 3640 GCCGGC [GA]^CCGG[CT]
4034 GCCGGC
NaeI 3640 GCCGGC GCC^GGC
4034 GCCGGC
NgoMIV 3640 GCCGGC G^CCGGC
4034 GCCGGC
PflMI 1902 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3840 CCACCTGGTGG
PvuII 321 CAGCTG CAG^CTG
2300 CAGCTG
RsaI 295 GTAC GT^AC
1606 GTAC
SapI 972 GCTCTTC GCTCTTC(1/4)
2368 GCTCTTC
SexAI 3203 ACCAGGT A^CC[AT]GGT
3842 ACCTGGT
SmaI 2020 CCCGGG CCC^GGG
2986 CCCGGG
SspI 1386 AATATT AAT^ATT
1451 AATATT
Van91I 1902 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3840 CCACCTGGTGG
XcmI 402 CCAAGCTTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3738 CCACGCTGTGCCTGG
XmaCI 2020 CCCGGG C^CCGGG
2986 CCCGGG
XmaI 2020 CCCGGG C^CCGGG
2986 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AflIII AluI Alw44I
AlwI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I