##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I AspI BamHI BbrPI BclI
BfrI BsaAI BsiWI BspDI BspHI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
MluI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI SalI SapI
SexAI SfiI SfuI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3565 GGTACC G^GTACC
AflIII 181 ACGTGT A^C[GA][CT]GT
AhdI 4419 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2103 GATCC GGATC(4/5)
Aor51HI 2373 AGCGCT AGC^GCT
Asp718I 3565 GGTACC G^GTACC
AspEI 4419 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1980 TGCGCA TGC^GCA
AvrII 476 CCTAGG C^CTAGG
BcgI 2757 GCAGCTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 392 GTATCC GTATCC(6/5)
BglII 3247 AGATCT A^GATCT
BlnI 476 CCTAGG C^CTAGG
BsaBI 3604 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1776 CGACCG CG[GA][CT]^CG
BsmBI 3138 GAGACG CGTCTC(1/5)
BsmI 4562 GCATTC GAATGC(1/-1)
Bsp1407I 775 TGTACA T^GTACA
BsrDI 1445 CATTGC GCAATG(2/0)
BsrGI 775 TGTACA T^GTACA
BstYI 3247 AGATCT [GA]^GATC[CT]
CpoI 2211 CGGTCCG CG^G[AT]CCG
Eam1105I 4419 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2373 AGCGCT AGC^GCT
FspI 1980 TGCGCA TGC^GCA
HincII 110 GTTGAC GT[CT]^[GA]AC
HindII 110 GTTGAC GT[CT]^[GA]AC
KpnI 3565 GGTACC GGTAC^C
MamI 3604 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3247 AGATCT [GA]^GATC[CT]
MslI 4349 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 2924 GCATGT [GA]CATG^[CT]
PaeR7I 2673 CTCGAG C^TCGAG
PshAI 3401 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2211 CGGTCCG CG^G[AT]CCG
SbfI 4234 CCTGCAGG CCTGCA^GG
SgrAI 753 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 4234 CCTGCAGG CCTGCA^GG
SspBI 775 TGTACA T^GTACA
TfiI 655 GAATC G^A[AT]TC
XhoI 2673 CTCGAG C^TCGAG
XhoII 3247 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 88 TCCGGA T^CCGGA
395 TCCGGA
BbeI 757 GGCGCC GGCGC^C
807 GGCGCC
BbsI 61 GAAGAC GAAGAC(2/6)
2252 GTCTTC
BbvCI 1561 CCTCAGC CCTCAGC(-5/-2)
4085 CCTCAGC
BpuAI 61 GAAGAC GAAGAC(2/6)
2252 GTCTTC
BseAI 88 TCCGGA T^CCGGA
395 TCCGGA
BspEI 88 TCCGGA T^CCGGA
395 TCCGGA
BssSI 3677 CACGAG CACGAG(-5/-1)
1541 CTCGTG
BstAPI 3784 GCAAGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3976 GCAGCACTTGC
DraI 562 TTTAAA TTT^AAA
3723 TTTAAA
EcoNI 1109 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2277 CCTGAGAGAGG
KasI 757 GGCGCC G^GCGCC
807 GGCGCC
MfeI 2836 CAATTG C^AATTG
4490 CAATTG
MroI 88 TCCGGA T^CCGGA
395 TCCGGA
MunI 2836 CAATTG C^AATTG
4490 CAATTG
NarI 757 GGCGCC GG^CGCC
807 GGCGCC
PflMI 3816 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3891 CCACTGCTTGG
SfoI 757 GGCGCC GGC^GCC
807 GGCGCC
SmaI 984 CCCGGG CCC^GGG
3433 CCCGGG
Van91I 3816 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3891 CCACTGCTTGG
XmaCI 984 CCCGGG C^CCGGG
3433 CCCGGG
XmaI 984 CCCGGG C^CCGGG
3433 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI