##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Aor51HI ApaI AscI Asp718I
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrDI BsrFI BssHII CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI FspI HaeII Hin1I HindIII
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I StuI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 1128 GCGG CCGC(-3/-1)
AclI 651 AACGTT AA^CGTT
AhdI 3623 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 932 GTGCAC G^TGCAC
AocI 3257 CCTGAGG CC^T[ACGT]AGG
ApaLI 932 GTGCAC G^TGCAC
AseI 4508 ATTAAT AT^TAAT
AsnI 4508 ATTAAT AT^TAAT
AspEI 3623 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2413 CTCGGG C^[CT]CG[GA]G
BalI 1973 TGGCCA TGG^CCA
BglI 1349 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 591 CGTCTC CGTCTC(1/5)
BsoBI 2413 CTCGGG C^[CT]CG[GA]G
Bst1107I 3302 GTATAC GTA^TAC
BstAPI 3720 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 792 TTCGAA TT^CGAA
BstZ17I 3302 GTATAC GTA^TAC
Bsu36I 3257 CCTGAGG CC^T[ACGT]AGG
CfoI 588 GCGC GCG^C
Eam1105I 3623 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3257 CCTGAGG CC^T[ACGT]AGG
EcoNI 247 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2381 GAATTC G^AATTC
FauI 1128 GCGGG CCCGC(4/6)
HgaI 590 GCGTC GACGC(5/10)
HhaI 588 GCGC GCG^C
HinP1I 588 GCGC G^CGC
HpaI 1851 GTTAAC GTT^AAC
MfeI 2724 CAATTG C^AATTG
MluNI 1973 TGGCCA TGG^CCA
MscI 1973 TGGCCA TGG^CCA
MunI 2724 CAATTG C^AATTG
NcoI 4431 CCATGG C^CATGG
NruI 1708 TCGCGA TCG^CGA
NspV 792 TTCGAA TT^CGAA
PflMI 4103 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4508 ATTAAT AT^TAAT
Psp1406I 651 AACGTT AA^CGTT
PstI 1799 CTGCAG CTGCA^G
SfuI 792 TTCGAA TT^CGAA
SnaBI 1893 TACGTA TAC^GTA
SspI 3427 AATATT AAT^ATT
Van91I 4103 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 589 CGCG CG^CG
1709 CGCG
AspHI 932 GTGCAC G[AT]GC[AT]^C
2192 GTGCTC
AspI 3747 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4169 GACTTTGTC
BanII 562 GGGCTC G[GA]GC[CT]^C
3711 GGGCTC
BbvCI 150 GCTGAGG CCTCAGC(-5/-2)
4078 GCTGAGG
BcgI 903 CGATTTATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4084 GCATGAGAATCG
BglII 2057 AGATCT A^GATCT
2543 AGATCT
BsaBI 2071 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2732 GATGAACATC
BsaI 2619 GAGACC GGTCTC(1/5)
3331 GGTCTC
BsiHKAI 932 GTGCAC G[AT]GC[AT]^C
2192 GTGCTC
Bsp1407I 1582 TGTACA T^GTACA
2816 TGTACA
BsrGI 1582 TGTACA T^GTACA
2816 TGTACA
BssSI 456 CACGAG CACGAG(-5/-1)
3842 CACGAG
BstEII 188 GGTCACC G^GT[ACGT]ACC
3699 GGTGACC
BstPI 188 GGTCACC G^GT[ACGT]ACC
3699 GGTGACC
BstUI 589 CGCG CG^CG
1709 CGCG
DsaI 1570 CCACGG C^C[GA][CT]GG
4431 CCATGG
EcoO65I 188 GGTCACC G^GT[ACGT]ACC
3699 GGTGACC
HincII 1851 GTTAAC GT[CT]^[GA]AC
2886 GTCAAC
HindII 1851 GTTAAC GT[CT]^[GA]AC
2886 GTCAAC
MamI 2071 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2732 GATGAACATC
MvnI 589 CGCG CG^CG
1709 CGCG
PflFI 3747 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4169 GACTTTGTC
SapI 4393 GAAGAGC GCTCTTC(1/4)
892 GCTCTTC
SmlI 519 CTCAAG C^T[CT][GA]AG
2801 CTTGAG
SspBI 1582 TGTACA T^GTACA
2816 TGTACA
SwaI 2043 ATTTAAAT ATTT^AAAT
4256 ATTTAAAT
Tth111I 3747 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4169 GACTTTGTC
XcmI 1499 CCAAATGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1576 CCACTATGTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI