##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII BcgI BclI BfrI BglII
BsaBI BseAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BstAPI BstBI ClaI CpoI
DraI DrdI EcoRI EcoRV FbaI
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1990 GTATAC GT^[AC][GT]AC
AcyI 765 GGCGCC G[GA]^CG[CT]C
AflIII 4160 ACGTGT A^C[GA][CT]GT
AocI 501 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2071 AGCGCT AGC^GCT
AspI 4128 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3990 CCTAGG C^CTAGG
BalI 2526 TGGCCA TGG^CCA
BamHI 647 GGATCC G^GATCC
BbeI 765 GGCGCC GGCGC^C
BbvCI 1806 CCTCAGC CCTCAGC(-5/-2)
BciVI 1070 GTATCC GTATCC(6/5)
BlnI 3990 CCTAGG C^CTAGG
BlpI 792 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 792 GCTCAGC GC^T[ACGT]AGC
BsaHI 765 GGCGCC G[GA]^CG[CT]C
BsiWI 206 CGTACG C^GTACG
BsrBI 566 GAGCGG CCGCTC(-3/-3)
BsrFI 2779 GCCGGT [GA]^CCGG[CT]
BssHII 3932 GCGCGC G^CGCGC
BssSI 555 CTCGTG CACGAG(-5/-1)
Bst1107I 1990 GTATAC GTA^TAC
BstZ17I 1990 GTATAC GTA^TAC
Bsu36I 501 CCTCAGG CC^T[ACGT]AGG
CelII 792 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2779 GCCGGT [GA]^CCGG[CT]
EagI 391 CGGCCG C^GGCCG
EclXI 391 CGGCCG C^GGCCG
Eco47III 2071 AGCGCT AGC^GCT
Eco52I 391 CGGCCG C^GGCCG
Eco81I 501 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1113 ATGCAT ATGCA^T
HgaI 3303 GCGTC GACGC(5/10)
Hin1I 765 GGCGCC G[GA]^CG[CT]C
HincII 1768 GTTGAC GT[CT]^[GA]AC
HindII 1768 GTTGAC GT[CT]^[GA]AC
HindIII 473 AAGCTT A^AGCTT
KasI 765 GGCGCC G^GCGCC
MluNI 2526 TGGCCA TGG^CCA
MscI 2526 TGGCCA TGG^CCA
NarI 765 GGCGCC GG^CGCC
NcoI 2846 CCATGG C^CATGG
NheI 493 GCTAGC G^CTAGC
NsiI 1113 ATGCAT ATGCA^T
PflFI 4128 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 861 CCTGCAGG CCTGCA^GG
SfoI 765 GGCGCC GGC^GCC
Sse8387I 861 CCTGCAGG CCTGCA^GG
SspI 1016 AATATT AAT^ATT
TfiI 2327 GAATC G^A[AT]TC
Tth111I 4128 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 133 AAATTT [GA]^AATT[CT]
1080 AAATTC
AlwNI 1809 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2241 CAGGGGCTG
ApoI 133 AAATTT [GA]^AATT[CT]
1080 AAATTC
Asp700I 2382 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2481 GAAGACGTTC
BsiEI 391 CGGCCG CG[GA][CT]^CG
3484 CGGTCG
BstEII 1185 GGTCACC G^GT[ACGT]ACC
2782 GGTGACC
BstPI 1185 GGTCACC G^GT[ACGT]ACC
2782 GGTGACC
EcoNI 620 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3419 CCTCCCCGAGG
EcoO65I 1185 GGTCACC G^GT[ACGT]ACC
2782 GGTGACC
HaeII 765 GGCGCC [GA]GCGC^[CT]
2071 AGCGCT
NspI 1115 GCATGT [GA]CATG^[CT]
3284 GCATGT
SacI 2064 GAGCTC GAGCT^C
3592 GAGCTC
XmnI 2382 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2481 GAAGACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
Alw44I AlwI ApaI ApaLI AspEI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII