##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AscI AseI AsnI
Asp700I Asp718I BbrPI BciVI BclI
BglI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI HindIII HpaI KpnI KspI
MamI MluI MroI MvnI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI StuI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2376 GTCTAC GT^[AC][GT]AC
AflII 2690 CTTAAG C^TTAAG
AhdI 2363 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 155 GTGCAC G^TGCAC
ApaLI 155 GTGCAC G^TGCAC
AspEI 2363 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 41 TGCGCA TGC^GCA
AvrII 2728 CCTAGG C^CTAGG
BalI 2970 TGGCCA TGG^CCA
BamHI 1906 GGATCC G^GATCC
BbsI 1978 GTCTTC GAAGAC(2/6)
BfrI 2690 CTTAAG C^TTAAG
BglII 2189 AGATCT A^GATCT
BlnI 2728 CCTAGG C^CTAGG
BpuAI 1978 GTCTTC GAAGAC(2/6)
BsmI 3700 GAATGC GAATGC(1/-1)
CpoI 3646 CGGTCCG CG^G[AT]CCG
DraI 3986 TTTAAA TTT^AAA
DraIII 2873 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2970 TGGCCA [CT]^GGCC[GA]
Eam1105I 2363 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 41 TGCGCA TGC^GCA
MfeI 1 CAATTG C^AATTG
MluNI 2970 TGGCCA TGG^CCA
MscI 2970 TGGCCA TGG^CCA
MunI 1 CAATTG C^AATTG
NaeI 3226 GCCGGC GCC^GGC
NdeI 4011 CATATG CA^TATG
NgoMIV 3226 GCCGGC G^CCGGC
PaeR7I 92 CTCGAG C^TCGAG
PshAI 2483 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3646 CGGTCCG CG^G[AT]CCG
SexAI 1041 ACCAGGT A^CC[AT]GGT
SmaI 525 CCCGGG CCC^GGG
SpeI 615 ACTAGT A^CTAGT
SspI 3862 AATATT AAT^ATT
XbaI 3669 TCTAGA T^CTAGA
XcmI 2758 CCATCCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 92 CTCGAG C^TCGAG
XmaCI 525 CCCGGG C^CCGGG
XmaI 525 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 1118 AGCGCT AGC^GCT
2402 AGCGCT
AspI 3135 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4088 GACTCTGTC
BbeI 2441 GGCGCC GGCGC^C
4053 GGCGCC
BcgI 165 GCAGCCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
462 GCACATGCATCG
BsrBI 1068 CCGCTC CCGCTC(-3/-3)
312 GAGCGG
BsrDI 1173 CATTGC GCAATG(2/0)
533 GCAATG
BstXI 369 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3890 CCAACACTTTGG
DrdI 1678 GACACCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3156 GACCTTCCAGTC
Eco47III 1118 AGCGCT AGC^GCT
2402 AGCGCT
HincII 1821 GTTGAC GT[CT]^[GA]AC
2480 GTTGAC
HindII 1821 GTTGAC GT[CT]^[GA]AC
2480 GTTGAC
KasI 2441 GGCGCC G^GCGCC
4053 GGCGCC
NarI 2441 GGCGCC GG^CGCC
4053 GGCGCC
NcoI 431 CCATGG C^CATGG
2502 CCATGG
PflFI 3135 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4088 GACTCTGTC
SacI 1432 GAGCTC GAGCT^C
3665 GAGCTC
SfoI 2441 GGCGCC GGC^GCC
4053 GGCGCC
SphI 2425 GCATGC GCATG^C
2823 GCATGC
Tth111I 3135 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4088 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII