##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspHI AspI AviII BamHI
BcgI BfrI BglI BglII BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI HpaI KpnI KspI
MamI MroI NotI NruI NspV
PacI PaeR7I PflFI PinAI Psp1406I
PvuI RsrII SacI SacII SbfI
SexAI SfiI SfuI SgrAI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 4334 GACGTC GACGT^C
AhdI 3548 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4105 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3548 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2444 GGCGCC GGCGC^C
BbrPI 1214 CACGTG CAC^GTG
BseRI 3884 GAGGAG GAGGAG(10/8)
Bsp1407I 1204 TGTACA T^GTACA
BspHI 1186 TCATGA T^CATGA
BsrFI 4079 GCCGGC [GA]^CCGG[CT]
BsrGI 1204 TGTACA T^GTACA
Cfr10I 4079 GCCGGC [GA]^CCGG[CT]
DrdI 2575 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3548 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3901 GAAGAG CTCTTC(1/4)
EcoRV 4183 GATATC GAT^ATC
HaeII 2444 GGCGCC [GA]GCGC^[CT]
KasI 2444 GGCGCC G^GCGCC
Ksp632I 3901 GAAGAG CTCTTC(1/4)
MfeI 2389 CAATTG C^AATTG
MluI 1660 ACGCGT A^CGCGT
MunI 2389 CAATTG C^AATTG
NaeI 4079 GCCGGC GCC^GGC
NarI 2444 GGCGCC GG^CGCC
NgoMIV 4079 GCCGGC G^CCGGC
NheI 3766 GCTAGC G^CTAGC
PflMI 4148 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1214 CACGTG CAC^GTG
PmeI 2300 GTTTAAAC GTTT^AAAC
PmlI 1214 CACGTG CAC^GTG
PpuMI 2505 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2133 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4329 CTGCAG CTGCA^G
RcaI 1186 TCATGA T^CATGA
SalI 3545 GTCGAC G^TCGAC
SapI 3901 GAAGAGC GCTCTTC(1/4)
SfoI 2444 GGCGCC GGC^GCC
SspBI 1204 TGTACA T^GTACA
Van91I 4148 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 4105 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1805 GTAGAC GT^[AC][GT]AC
3545 GTCGAC
AccII 1661 CGCG CG^CG
4602 CGCG
AlwNI 1680 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2029 CAGCTTCTG
ApaI 2711 GGGCCC GGGCC^C
3838 GGGCCC
AvaII 2506 GGTCC G^G[AT]CC
2893 GGACC
AvrII 1610 CCTAGG C^CTAGG
1969 CCTAGG
BanI 2444 GGCGCC G^G[CT][GA]CC
3310 GGCACC
BbsI 2597 GAAGAC GAAGAC(2/6)
2584 GTCTTC
BciVI 169 GTATCC GTATCC(6/5)
2965 GTATCC
BlnI 1610 CCTAGG C^CTAGG
1969 CCTAGG
BpuAI 2597 GAAGAC GAAGAC(2/6)
2584 GTCTTC
BsaI 1464 GGTCTC GGTCTC(1/5)
4761 GGTCTC
BspLU11I 2624 ACATGT A^CATGT
3331 ACATGT
BspMI 2350 GCAGGT ACCTGC(4/8)
4821 GCAGGT
BstAPI 1408 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3026 GCACTATGTGC
BstUI 1661 CGCG CG^CG
4602 CGCG
BstYI 3240 AGATCC [GA]^GATC[CT]
3477 GGATCT
EcoT22I 1166 ATGCAT ATGCA^T
2469 ATGCAT
HgaI 3876 GACGC GACGC(5/10)
3636 GCGTC
HincII 1794 GTTGAC GT[CT]^[GA]AC
3545 GTCGAC
HindII 1794 GTTGAC GT[CT]^[GA]AC
3545 GTCGAC
HindIII 2686 AAGCTT A^AGCTT
4225 AAGCTT
MflI 3240 AGATCC [GA]^GATC[CT]
3477 GGATCT
MvnI 1661 CGCG CG^CG
4602 CGCG
NcoI 2114 CCATGG C^CATGG
3498 CCATGG
NsiI 1166 ATGCAT ATGCA^T
2469 ATGCAT
ScaI 709 AGTACT AGT^ACT
1338 AGTACT
SfcI 1013 CTATAG C^T[GA][CT]AG
4329 CTGCAG
SmaI 3848 CCCGGG CCC^GGG
4632 CCCGGG
SnaBI 528 TACGTA TAC^GTA
2167 TACGTA
SspI 1132 AATATT AAT^ATT
1348 AATATT
StuI 1817 AGGCCT AGG^CCT
3321 AGGCCT
XhoII 3240 AGATCC [GA]^GATC[CT]
3477 GGATCT
XmaCI 3848 CCCGGG C^CCGGG
4632 CCCGGG
XmaI 3848 CCCGGG C^CCGGG
4632 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaI BalI BanII BbvCI BbvI
BclI BcnI BfaI BmyI BpmI
BsaAI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I