##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BcnI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstUI
BstZ17I Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI FspI Hin1I
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflII 2291 CTTAAG C^TTAAG
Alw44I 1794 GTGCAC G^TGCAC
AlwNI 1325 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2733 CCTGAGG CC^T[ACGT]AGG
ApaLI 1794 GTGCAC G^TGCAC
AseI 4126 ATTAAT AT^TAAT
AsnI 4126 ATTAAT AT^TAAT
BalI 1935 TGGCCA TGG^CCA
BamHI 3984 GGATCC G^GATCC
BanII 457 GAGCTC G[GA]GC[CT]^C
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BcgI 381 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 147 GTATCC GTATCC(6/5)
BclI 3613 TGATCA T^GATCA
BfrI 2291 CTTAAG C^TTAAG
BpmI 774 CTGGAG CTGGAG(16/14)
BsaAI 1942 CACGTA [CT]AC^GT[GA]
BsaI 1355 GAGACC GGTCTC(1/5)
BspHI 2842 TCATGA T^CATGA
BspMI 377 ACCTGC ACCTGC(4/8)
BstAPI 1739 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2525 TTCGAA TT^CGAA
BstEII 3308 GGTGACC G^GT[ACGT]ACC
BstPI 3308 GGTGACC G^GT[ACGT]ACC
BstXI 1904 CCAAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2733 CCTGAGG CC^T[ACGT]AGG
CfoI 1327 GCGC GCG^C
DraII 392 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1935 TGGCCA [CT]^GGCC[GA]
Eco81I 2733 CCTGAGG CC^T[ACGT]AGG
EcoNI 399 CCTATATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 392 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3308 GGTGACC G^GT[ACGT]ACC
EcoT22I 477 ATGCAT ATGCA^T
FauI 1310 GCGGG CCCGC(4/6)
FbaI 3613 TGATCA T^GATCA
HaeII 1326 AGCGCC [GA]GCGC^[CT]
HapII 1237 CCGG C^CGG
HhaI 1327 GCGC GCG^C
HinP1I 1327 GCGC G^CGC
HindIII 3880 AAGCTT A^AGCTT
HpaI 4253 GTTAAC GTT^AAC
HpaII 1237 CCGG C^CGG
MaeII 1943 ACGT A^CGT
MluNI 1935 TGGCCA TGG^CCA
MscI 1935 TGGCCA TGG^CCA
MspI 1237 CCGG C^CGG
NsiI 477 ATGCAT ATGCA^T
NspV 2525 TTCGAA TT^CGAA
PpuMI 392 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4126 ATTAAT AT^TAAT
RcaI 2842 TCATGA T^CATGA
SacI 457 GAGCTC GAGCT^C
SbfI 69 CCTGCAGG CCTGCA^GG
SexAI 3312 ACCTGGT A^CC[AT]GGT
SfuI 2525 TTCGAA TT^CGAA
Sse8387I 69 CCTGCAGG CCTGCA^GG
XcmI 844 CCAGGAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2385 GTCTAC GT^[AC][GT]AC
2962 GTCTAC
AflIII 1714 ACATGT A^C[GA][CT]GT
1949 ACATGT
Asp700I 2910 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2940 GAAGGCTTTC
AvaII 393 GGTCC G^G[AT]CC
745 GGACC
BsmI 3921 GAATGC GAATGC(1/-1)
1083 GCATTC
Bsp1407I 1132 TGTACA T^GTACA
3885 TGTACA
BspLU11I 1714 ACATGT A^CATGT
1949 ACATGT
BsrGI 1132 TGTACA T^GTACA
3885 TGTACA
BstYI 1069 AGATCC [GA]^GATC[CT]
3984 GGATCC
DrdI 991 GACAGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2822 GACTTCCGTGTC
HgaI 961 GCGTC GACGC(5/10)
2960 GCGTC
HincII 3954 GTTGAC GT[CT]^[GA]AC
4253 GTTAAC
HindII 3954 GTTGAC GT[CT]^[GA]AC
4253 GTTAAC
MflI 1069 AGATCC [GA]^GATC[CT]
3984 GGATCC
PflMI 3271 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4412 CCAGGATATGG
PleI 53 GACTC GAGTC(4/5)
159 GAGTC
PstI 70 CTGCAG CTGCA^G
247 CTGCAG
PvuII 921 CAGCTG CAG^CTG
993 CAGCTG
ScaI 1569 AGTACT AGT^ACT
2135 AGTACT
SmlI 2291 CTTAAG C^T[CT][GA]AG
3657 CTTGAG
SspBI 1132 TGTACA T^GTACA
3885 TGTACA
StuI 213 AGGCCT AGG^CCT
1183 AGGCCT
Van91I 3271 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4412 CCAGGATATGG
XbaI 1090 TCTAGA T^CTAGA
1492 TCTAGA
XhoII 1069 AGATCC [GA]^GATC[CT]
3984 GGATCC
XmnI 2910 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2940 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BfaI
BlpI BmyI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I