##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI Asp700I AviII BcgI
BfrI BsaBI BsiWI BsmBI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HindIII HpaI KspI MamI
MfeI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
SacII ScaI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2837 GACGTC GACGT^C
AhdI 7495 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7495 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4013 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 6779 GGATCC G^GATCC
BbeI 3611 GGCGCC GGCGC^C
BclI 7205 TGATCA T^GATCA
BglII 72 AGATCT A^GATCT
BspLU11I 6229 ACATGT A^CATGT
BstEII 678 GGTCACC G^GT[ACGT]ACC
BstPI 678 GGTCACC G^GT[ACGT]ACC
CpoI 1725 CGGACCG CG^G[AT]CCG
DraIII 896 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 7495 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 678 GGTCACC G^GT[ACGT]ACC
EcoRI 5633 GAATTC G^AATTC
FbaI 7205 TGATCA T^GATCA
KasI 3611 GGCGCC G^GCGCC
MluI 4306 ACGCGT A^CGCGT
NarI 3611 GGCGCC GG^CGCC
NheI 1131 GCTAGC G^CTAGC
PflFI 4013 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1725 CGGACCG CG^G[AT]CCG
SexAI 6064 ACCTGGT A^CC[AT]GGT
SfiI 1810 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3611 GGCGCC GGC^GCC
SmaI 2615 CCCGGG CCC^GGG
SphI 7266 GCATGC GCATG^C
Tth111I 4013 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5376 TCTAGA T^CTAGA
XmaCI 2615 CCCGGG C^CCGGG
XmaI 2615 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 3451 TCCGGA T^CCGGA
6287 TCCGGA
AvrII 1114 CCTAGG C^CTAGG
7254 CCTAGG
BbrPI 2273 CACGTG CAC^GTG
4469 CACGTG
BciVI 4942 GTATCC GTATCC(6/5)
5673 GTATCC
BlnI 1114 CCTAGG C^CTAGG
7254 CCTAGG
BsaWI 3451 TCCGGA [AT]^CCGG[AT]
6287 TCCGGA
BseAI 3451 TCCGGA T^CCGGA
6287 TCCGGA
BsiEI 3277 CGGTCG CG[GA][CT]^CG
3544 CGGTCG
BspEI 3451 TCCGGA T^CCGGA
6287 TCCGGA
BspHI 4111 TCATGA T^CATGA
7461 TCATGA
BsrFI 3275 GCCGGT [GA]^CCGG[CT]
4425 ACCGGC
Cfr10I 3275 GCCGGT [GA]^CCGG[CT]
4425 ACCGGC
EcoT22I 16 ATGCAT ATGCA^T
7449 ATGCAT
MroI 3451 TCCGGA T^CCGGA
6287 TCCGGA
NsiI 16 ATGCAT ATGCA^T
7449 ATGCAT
PmaCI 2273 CACGTG CAC^GTG
4469 CACGTG
PmlI 2273 CACGTG CAC^GTG
4469 CACGTG
PshAI 2923 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5740 GACTGCTGTC
RcaI 4111 TCATGA T^CATGA
7461 TCATGA
SbfI 3113 CCTGCAGG CCTGCA^GG
4220 CCTGCAGG
Sse8387I 3113 CCTGCAGG CCTGCA^GG
4220 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SalI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII