##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AcsI AflII
AgeI ApoI AscI AseI AsnI
AspI AvrII BciVI BclI BfrI
BglII BlnI BseAI BsiWI BsmI
BspEI BspLU11I BspMI Bst1107I BstZ17I
DraI DraIII EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KspI MfeI
MluI MroI MseI MunI NdeI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
SacII SalI SbfI ScaI SfiI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2676 GGTACC G^GTACC
AclI 679 AACGTT AA^CGTT
AflIII 3138 ACACGT A^C[GA][CT]GT
Alw44I 1009 GTGCAC G^TGCAC
AocI 454 CCTGAGG CC^T[ACGT]AGG
ApaLI 1009 GTGCAC G^TGCAC
Asp700I 2073 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2676 GGTACC G^GTACC
BamHI 618 GGATCC G^GATCC
BbsI 1774 GTCTTC GAAGAC(2/6)
BbvCI 3012 GCTGAGG CCTCAGC(-5/-2)
BcgI 1885 CGACTGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1774 GTCTTC GAAGAC(2/6)
BsaWI 648 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2215 TGTACA T^GTACA
BspDI 1606 ATCGAT AT^CGAT
BspHI 2069 TCATGA T^CATGA
BsrGI 2215 TGTACA T^GTACA
BstAPI 2793 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1777 TTCGAA TT^CGAA
Bsu36I 454 CCTGAGG CC^T[ACGT]AGG
ClaI 1606 ATCGAT AT^CGAT
CpoI 2543 CGGTCCG CG^G[AT]CCG
DrdI 3051 GACGCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 454 CCTGAGG CC^T[ACGT]AGG
FseI 1110 GGCCGGCC GGCCGG^CC
HincII 3068 GTCAAC GT[CT]^[GA]AC
HindII 3068 GTCAAC GT[CT]^[GA]AC
KpnI 2676 GGTACC GGTAC^C
MslI 591 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 574 GCTAGC G^CTAGC
NspV 1777 TTCGAA TT^CGAA
PflMI 2718 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 940 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 679 AACGTT AA^CGTT
RcaI 2069 TCATGA T^CATGA
RsrII 2543 CGGTCCG CG^G[AT]CCG
SacI 2910 GAGCTC GAGCT^C
SfuI 1777 TTCGAA TT^CGAA
SgrAI 1307 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2202 GCATGC GCATG^C
SspBI 2215 TGTACA T^GTACA
StuI 2054 AGGCCT AGG^CCT
Van91I 2718 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2073 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 85 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
654 GACTGCTGGTC
AspEI 85 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
654 GACTGCTGGTC
AviII 1446 TGCGCA TGC^GCA
2444 TGCGCA
BbeI 1101 GGCGCC GGCGC^C
1305 GGCGCC
BbrPI 595 CACGTG CAC^GTG
3139 CACGTG
BfaI 575 CTAG C^TAG
764 CTAG
BsaAI 595 CACGTG [CT]AC^GT[GA]
3139 CACGTG
BsaBI 619 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3160 GATAGCCATC
BsmBI 1672 CGTCTC CGTCTC(1/5)
2326 CGTCTC
BsrDI 2106 GCAATG GCAATG(2/0)
2945 GCAATG
BssSI 1624 CTCGTG CACGAG(-5/-1)
2812 CTCGTG
BstEII 870 GGTAACC G^GT[ACGT]ACC
2158 GGTTACC
BstPI 870 GGTAACC G^GT[ACGT]ACC
2158 GGTTACC
Eam1105I 85 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
654 GACTGCTGGTC
EcoNI 1653 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2115 CCTGGACCAGG
EcoO65I 870 GGTAACC G^GT[ACGT]ACC
2158 GGTTACC
FspI 1446 TGCGCA TGC^GCA
2444 TGCGCA
KasI 1101 GGCGCC G^GCGCC
1305 GGCGCC
MaeI 575 CTAG C^TAG
764 CTAG
MamI 619 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3160 GATAGCCATC
NaeI 1111 GCCGGC GCC^GGC
1308 GCCGGC
NarI 1101 GGCGCC GG^CGCC
1305 GGCGCC
NgoMIV 1111 GCCGGC G^CCGGC
1308 GCCGGC
NspI 1849 GCATGT [GA]CATG^[CT]
2202 GCATGC
PmaCI 595 CACGTG CAC^GTG
3139 CACGTG
PmlI 595 CACGTG CAC^GTG
3139 CACGTG
SapI 2491 GAAGAGC GCTCTTC(1/4)
1125 GCTCTTC
SexAI 668 ACCAGGT A^CC[AT]GGT
2120 ACCAGGT
SfoI 1101 GGCGCC GGC^GCC
1305 GGCGCC
SmlI 694 CTCAAG C^T[CT][GA]AG
1833 CTCAAG
Tsp45I 312 GTCAC ^GT[GC]AC
1519 GTGAC
Tsp509I 953 AATT ^AATT
2220 AATT
XcmI 1454 CCAAGGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2129 CCATGGTGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI TspRI TthHB8I XhoII XmaCI
XmaI