##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AviII BbeI BcgI BclI BglII
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI Eco47III EcoRV
FbaI FseI FspI KasI KspI
MfeI MluI MunI NarI NheI
NotI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3381 GTAGAC GT^[AC][GT]AC
AccII 2286 CGCG CG^CG
AhdI 1981 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 714 ATTAAT AT^TAAT
AsnI 714 ATTAAT AT^TAAT
AspEI 1981 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsaBI 3617 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1102 TCATGA T^CATGA
BspLU11I 595 ACATGT A^CATGT
BstAPI 3018 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2286 CGCG CG^CG
DrdI 1966 GACAGGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1981 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 5136 AGCGCC [GA]GCGC^[CT]
HincII 649 GTTAAC GT[CT]^[GA]AC
HindII 649 GTTAAC GT[CT]^[GA]AC
HindIII 5833 AAGCTT A^AGCTT
HpaI 649 GTTAAC GTT^AAC
MamI 3617 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2286 CGCG CG^CG
NdeI 588 CATATG CA^TATG
NruI 2285 TCGCGA TCG^CGA
PshBI 714 ATTAAT AT^TAAT
RcaI 1102 TCATGA T^CATGA
SbfI 1487 CCTGCAGG CCTGCA^GG
ScaI 4360 AGTACT AGT^ACT
SexAI 5338 ACCTGGT A^CC[AT]GGT
SphI 5130 GCATGC GCATG^C
Sse8387I 1487 CCTGCAGG CCTGCA^GG
XbaI 5473 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2169 GGTACC G^GTACC
3996 GGTACC
AccIII 1611 TCCGGA T^CCGGA
2425 TCCGGA
AflII 2152 CTTAAG C^TTAAG
2939 CTTAAG
Alw44I 3393 GTGCAC G^TGCAC
3939 GTGCAC
ApaLI 3393 GTGCAC G^TGCAC
3939 GTGCAC
Asp700I 3245 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5542 GAACAGATTC
Asp718I 2169 GGTACC G^GTACC
3996 GGTACC
BbrPI 1108 CACGTG CAC^GTG
1685 CACGTG
BbvCI 2173 CCTCAGC CCTCAGC(-5/-2)
3678 GCTGAGG
BfrI 2152 CTTAAG C^TTAAG
2939 CTTAAG
BseAI 1611 TCCGGA T^CCGGA
2425 TCCGGA
Bsp1407I 592 TGTACA T^GTACA
598 TGTACA
BspEI 1611 TCCGGA T^CCGGA
2425 TCCGGA
BsrDI 924 GCAATG GCAATG(2/0)
4094 GCAATG
BsrGI 592 TGTACA T^GTACA
598 TGTACA
BssSI 175 CACGAG CACGAG(-5/-1)
3232 CTCGTG
BstEII 3684 GGTCACC G^GT[ACGT]ACC
4045 GGTGACC
BstPI 3684 GGTCACC G^GT[ACGT]ACC
4045 GGTGACC
EagI 1567 CGGCCG C^GGCCG
4624 CGGCCG
EclXI 1567 CGGCCG C^GGCCG
4624 CGGCCG
Eco52I 1567 CGGCCG C^GGCCG
4624 CGGCCG
EcoO65I 3684 GGTCACC G^GT[ACGT]ACC
4045 GGTGACC
EcoRI 375 GAATTC G^AATTC
1059 GAATTC
EcoT22I 789 ATGCAT ATGCA^T
6349 ATGCAT
KpnI 2169 GGTACC GGTAC^C
3996 GGTACC
MroI 1611 TCCGGA T^CCGGA
2425 TCCGGA
NaeI 1565 GCCGGC GCC^GGC
3337 GCCGGC
NgoMIV 1565 GCCGGC G^CCGGC
3337 GCCGGC
NsiI 789 ATGCAT ATGCA^T
6349 ATGCAT
PaeR7I 1178 CTCGAG C^TCGAG
5537 CTCGAG
PmaCI 1108 CACGTG CAC^GTG
1685 CACGTG
PmlI 1108 CACGTG CAC^GTG
1685 CACGTG
SapI 1655 GAAGAGC GCTCTTC(1/4)
3843 GCTCTTC
SmaI 14 CCCGGG CCC^GGG
3485 CCCGGG
SspBI 592 TGTACA T^GTACA
598 TGTACA
TaqI 1179 TCGA T^CGA
5538 TCGA
TthHB8I 1179 TCGA T^CGA
5538 TCGA
XhoI 1178 CTCGAG C^TCGAG
5537 CTCGAG
XmaCI 14 CCCGGG C^CCGGG
3485 CCCGGG
XmaI 14 CCCGGG C^CCGGG
3485 CCCGGG
XmnI 3245 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5542 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII