##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BclI BfrI BlnI
BsaBI BsiWI BsmI BspDI BspHI
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SpeI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1565 GACGTC GACGT^C
AcsI 6401 AAATTT [GA]^AATT[CT]
ApoI 6401 AAATTT [GA]^AATT[CT]
BbrPI 2963 CACGTG CAC^GTG
BciVI 3952 GTATCC GTATCC(6/5)
BglII 479 AGATCT A^GATCT
BlpI 1366 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC
BsiEI 5054 CGGCCG CG[GA][CT]^CG
Bsp1407I 6382 TGTACA T^GTACA
BspLU11I 1443 ACATGT A^CATGT
BsrGI 6382 TGTACA T^GTACA
BssHII 800 GCGCGC G^CGCGC
CelII 1366 GCTGAGC GC^T[ACGT]AGC
DraI 6398 TTTAAA TTT^AAA
DrdI 37 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5054 CGGCCG C^GGCCG
EclXI 5054 CGGCCG C^GGCCG
Eco52I 5054 CGGCCG C^GGCCG
EcoNI 1771 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 6099 CCGCGG CCGC^GG
NcoI 1528 CCATGG C^CATGG
PaeR7I 1205 CTCGAG C^TCGAG
PmaCI 2963 CACGTG CAC^GTG
PmlI 2963 CACGTG CAC^GTG
PshAI 443 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 548 GAGCTC GAGCT^C
SacII 6099 CCGCGG CCGC^GG
SnaBI 2567 TACGTA TAC^GTA
SphI 113 GCATGC GCATG^C
SspBI 6382 TGTACA T^GTACA
SspI 6394 AATATT AAT^ATT
SwaI 6397 ATTTAAAT ATTT^AAAT
Tsp509I 6402 AATT ^AATT
XhoI 1205 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 2658 TCCGGA T^CCGGA
3286 TCCGGA
AocI 205 CCTGAGG CC^T[ACGT]AGG
3728 CCTGAGG
Aor51HI 699 AGCGCT AGC^GCT
1164 AGCGCT
BamHI 3144 GGATCC G^GATCC
3446 GGATCC
BcgI 5659 CGACCTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1764 GCAAGATCCTCG
BsaWI 2658 TCCGGA [AT]^CCGG[AT]
3286 TCCGGA
BseAI 2658 TCCGGA T^CCGGA
3286 TCCGGA
BspEI 2658 TCCGGA T^CCGGA
3286 TCCGGA
BsrDI 5929 CATTGC GCAATG(2/0)
2085 GCAATG
BstEII 1412 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
BstPI 1412 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
Bsu36I 205 CCTGAGG CC^T[ACGT]AGG
3728 CCTGAGG
DraIII 1337 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4669 CACCGAGTG
Eco47III 699 AGCGCT AGC^GCT
1164 AGCGCT
Eco81I 205 CCTGAGG CC^T[ACGT]AGG
3728 CCTGAGG
EcoO65I 1412 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
EcoT22I 1887 ATGCAT ATGCA^T
3427 ATGCAT
HindIII 2107 AAGCTT A^AGCTT
4501 AAGCTT
MroI 2658 TCCGGA T^CCGGA
3286 TCCGGA
NsiI 1887 ATGCAT ATGCA^T
3427 ATGCAT
SapI 3358 GAAGAGC GCTCTTC(1/4)
4959 GCTCTTC
SfiI 3050 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5941 GGCCCGCCAGGCC
SgrAI 3454 CGCCGGCG C[GA]^CCGG[CT]G
5074 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
ApaI ApaLI AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I SbfI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI