##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AcsI AgeI AhdI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI BciVI BclI BsiWI
BspDI BspHI BspLU11I Bst1107I BstZ17I
ClaI DraI DraIII Eam1105I EcoRI
EcoRV FbaI FseI HindIII HpaI
KpnI KspI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RcaI SacI SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SnaBI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 5165 TCCGGA T^CCGGA
AclI 3979 AACGTT AA^CGTT
AflII 2124 CTTAAG C^TTAAG
Alw44I 1627 GTGCAC G^TGCAC
ApaLI 1627 GTGCAC G^TGCAC
AspI 2450 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1213 TGCGCA TGC^GCA
BamHI 5079 GGATCC G^GATCC
BbrPI 699 CACGTG CAC^GTG
BfrI 2124 CTTAAG C^TTAAG
BglII 5703 AGATCT A^GATCT
BsaBI 5074 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5165 TCCGGA T^CCGGA
BsiEI 4778 CGGCCG CG[GA][CT]^CG
BsmI 3989 GCATTC GAATGC(1/-1)
Bsp1407I 4836 TGTACA T^GTACA
BspEI 5165 TCCGGA T^CCGGA
BsrGI 4836 TGTACA T^GTACA
BssHII 3824 GCGCGC G^CGCGC
BstBI 3983 TTCGAA TT^CGAA
EagI 4778 CGGCCG C^GGCCG
EclXI 4778 CGGCCG C^GGCCG
Eco52I 4778 CGGCCG C^GGCCG
EcoT22I 5961 ATGCAT ATGCA^T
FspI 1213 TGCGCA TGC^GCA
HincII 3416 GTCAAC GT[CT]^[GA]AC
HindII 3416 GTCAAC GT[CT]^[GA]AC
MamI 5074 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5165 TCCGGA T^CCGGA
NsiI 5961 ATGCAT ATGCA^T
NspV 3983 TTCGAA TT^CGAA
PflFI 2450 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 699 CACGTG CAC^GTG
PmlI 699 CACGTG CAC^GTG
Psp1406I 3979 AACGTT AA^CGTT
SfuI 3983 TTCGAA TT^CGAA
SpeI 3527 ACTAGT A^CTAGT
SspBI 4836 TGTACA T^GTACA
Tth111I 2450 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AvrII 3321 CCTAGG C^CTAGG
6167 CCTAGG
BlnI 3321 CCTAGG C^CTAGG
6167 CCTAGG
BsrDI 4915 CATTGC GCAATG(2/0)
1 GCAATG
BstEII 1488 GGTCACC G^GT[ACGT]ACC
3716 GGTGACC
BstPI 1488 GGTCACC G^GT[ACGT]ACC
3716 GGTGACC
CpoI 4651 CGGTCCG CG^G[AT]CCG
4863 CGGACCG
EcoO65I 1488 GGTCACC G^GT[ACGT]ACC
3716 GGTGACC
NcoI 5424 CCATGG C^CATGG
5895 CCATGG
RsrII 4651 CGGTCCG CG^G[AT]CCG
4863 CGGACCG
SapI 3264 GAAGAGC GCTCTTC(1/4)
5626 GAAGAGC
SphI 3490 GCATGC GCATG^C
4699 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI