##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AclI AflIII AhdI AscI
AseI AsnI AspEI AspI AvrII
BalI BamHI BbrPI BbsI BciVI
BclI BglII BlnI BpuAI BsaBI
BsmBI BspDI BspHI BspLU11I BssSI
Bst1107I BstZ17I ClaI CpoI Eam1105I
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MamI MluI MluNI
MscI NdeI NheI NsiI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4110 GACGTC GACGT^C
Acc65I 1558 GGTACC G^GTACC
AccIII 340 TCCGGA T^CCGGA
AgeI 1796 ACCGGT A^CCGGT
Aor51HI 4241 AGCGCT AGC^GCT
Asp718I 1558 GGTACC G^GTACC
AviII 1827 TGCGCA TGC^GCA
BbvCI 2417 GCTGAGG CCTCAGC(-5/-2)
BcgI 482 GCAAGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 340 TCCGGA T^CCGGA
BsiWI 2574 CGTACG C^GTACG
BsmI 293 GAATGC GAATGC(1/-1)
Bsp1407I 2112 TGTACA T^GTACA
BspEI 340 TCCGGA T^CCGGA
BsrDI 4567 GCAATG GCAATG(2/0)
BsrGI 2112 TGTACA T^GTACA
BstAPI 3430 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2009 TTCGAA TT^CGAA
BstEII 1926 GGTGACC G^GT[ACGT]ACC
BstPI 1926 GGTGACC G^GT[ACGT]ACC
DraIII 578 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 778 GACCCGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4678 CGGCCG C^GGCCG
EclXI 4678 CGGCCG C^GGCCG
Eco47III 4241 AGCGCT AGC^GCT
Eco52I 4678 CGGCCG C^GGCCG
EcoO65I 1926 GGTGACC G^GT[ACGT]ACC
EcoRI 857 GAATTC G^AATTC
FspI 1827 TGCGCA TGC^GCA
HindIII 598 AAGCTT A^AGCTT
KpnI 1558 GGTACC GGTAC^C
KspI 1760 CCGCGG CCGC^GG
MfeI 881 CAATTG C^AATTG
MroI 340 TCCGGA T^CCGGA
MunI 881 CAATTG C^AATTG
NotI 4677 GCGGCCGC GC^GGCCGC
NruI 1585 TCGCGA TCG^CGA
NspV 2009 TTCGAA TT^CGAA
PinAI 1796 ACCGGT A^CCGGT
SacII 1760 CCGCGG CCGC^GG
SapI 3808 GAAGAGC GCTCTTC(1/4)
SexAI 4393 ACCAGGT A^CC[AT]GGT
SfaNI 2178 GCATC GCATC(5/9)
SfuI 2009 TTCGAA TT^CGAA
SgrAI 1831 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 542 TACGTA TAC^GTA
SspBI 2112 TGTACA T^GTACA
XbaI 138 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 857 GAATTC [GA]^AATT[CT]
3509 GAATTT
Alw44I 985 GTGCAC G^TGCAC
3405 GTGCAC
AlwNI 1045 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1111 CAGGTGCTG
AocI 2734 CCTCAGG CC^T[ACGT]AGG
3696 CCTTAGG
ApaLI 985 GTGCAC G^TGCAC
3405 GTGCAC
ApoI 857 GAATTC [GA]^AATT[CT]
3509 GAATTT
Asp700I 332 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3899 GAAGGGTTTC
BlpI 636 GCTAAGC GC^T[ACGT]AGC
3572 GCTTAGC
Bpu1102I 636 GCTAAGC GC^T[ACGT]AGC
3572 GCTTAGC
BsaAI 542 TACGTA [CT]AC^GT[GA]
3424 CACGTA
BstXI 3877 CCACAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4487 CCAGCACCGTGG
BstYI 135 GGATCT [GA]^GATC[CT]
3692 AGATCC
Bsu36I 2734 CCTCAGG CC^T[ACGT]AGG
3696 CCTTAGG
CelII 636 GCTAAGC GC^T[ACGT]AGC
3572 GCTTAGC
Eco81I 2734 CCTCAGG CC^T[ACGT]AGG
3696 CCTTAGG
MflI 135 GGATCT [GA]^GATC[CT]
3692 AGATCC
PflMI 739 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
790 CCAGAGAATGG
PpuMI 1785 GGGTCCT [GA]G^G[AT]CC[CT]
4162 GGGTCCC
PshAI 1165 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3199 GACGGGAGTC
PstI 4175 CTGCAG CTGCA^G
4515 CTGCAG
SacI 93 GAGCTC GAGCT^C
4440 GAGCTC
SfcI 4175 CTGCAG C^T[GA][CT]AG
4515 CTGCAG
Van91I 739 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
790 CCAGAGAATGG
XhoII 135 GGATCT [GA]^GATC[CT]
3692 AGATCC
XmnI 332 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3899 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI AlwI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI