##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
BamHI BcgI BciVI BclI BfrI
BglII BsaBI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstAPI BstBI BstYI BstZ17I
ClaI CpoI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MflI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AlwI 2659 GGATC GGATC(4/5)
Aor51HI 2510 AGCGCT AGC^GCT
AspI 408 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4106 TGCGCA TGC^GCA
AvrII 655 CCTAGG C^CTAGG
BbrPI 663 CACGTG CAC^GTG
BlnI 655 CCTAGG C^CTAGG
BlpI 2336 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2336 GCTGAGC GC^T[ACGT]AGC
BsaAI 663 CACGTG [CT]AC^GT[GA]
BsaWI 2367 TCCGGT [AT]^CCGG[AT]
BsmBI 2132 CGTCTC CGTCTC(1/5)
BsmI 1608 GCATTC GAATGC(1/-1)
Bsp1407I 17 TGTACA T^GTACA
BsrBI 647 CCGCTC CCGCTC(-3/-3)
BsrGI 17 TGTACA T^GTACA
CelII 2336 GCTGAGC GC^T[ACGT]AGC
DpnII 2660 GATC ^GATC
DraI 1845 TTTAAA TTT^AAA
Eco47III 2510 AGCGCT AGC^GCT
FspI 4106 TGCGCA TGC^GCA
MaeII 664 ACGT A^CGT
MboI 2660 GATC ^GATC
NcoI 3053 CCATGG C^CATGG
NdeII 2660 GATC ^GATC
PaeR7I 908 CTCGAG C^TCGAG
PflFI 408 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 663 CACGTG CAC^GTG
PmlI 663 CACGTG CAC^GTG
Sau3AI 2660 GATC ^GATC
ScaI 2993 AGTACT AGT^ACT
SexAI 3728 ACCAGGT A^CC[AT]GGT
SphI 3963 GCATGC GCATG^C
SspBI 17 TGTACA T^GTACA
TaqI 909 TCGA T^CGA
Tth111I 408 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 909 TCGA T^CGA
XhoI 908 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 242 AAATTC [GA]^AATT[CT]
3149 AAATTC
AocI 3186 CCTGAGG CC^T[ACGT]AGG
3391 CCTGAGG
ApoI 242 AAATTC [GA]^AATT[CT]
3149 AAATTC
BsaI 2387 GGTCTC GGTCTC(1/5)
3533 GGTCTC
BssSI 76 CACGAG CACGAG(-5/-1)
748 CACGAG
BstEII 271 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
BstPI 271 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
Bsu36I 3186 CCTGAGG CC^T[ACGT]AGG
3391 CCTGAGG
DrdI 48 GACGGCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
128 GACGCCCAGGTC
Eco81I 3186 CCTGAGG CC^T[ACGT]AGG
3391 CCTGAGG
EcoO65I 271 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
KspI 2617 CCGCGG CCGC^GG
2918 CCGCGG
MseI 1166 TTAA T^TAA
1846 TTAA
MslI 2078 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2838 CACCAGGGTG
NaeI 3180 GCCGGC GCC^GGC
3912 GCCGGC
NgoMIV 3180 GCCGGC G^CCGGC
3912 GCCGGC
NspI 2083 GCATGT [GA]CATG^[CT]
3963 GCATGC
SacII 2617 CCGCGG CCGC^GG
2918 CCGCGG
SapI 1764 GAAGAGC GCTCTTC(1/4)
3015 GCTCTTC
SbfI 1799 CCTGCAGG CCTGCA^GG
3428 CCTGCAGG
SfiI 1669 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2811 GGCCCAGGTGGCC
SmlI 489 CTCAAG C^T[CT][GA]AG
908 CTCGAG
Sse8387I 1799 CCTGCAGG CCTGCA^GG
3428 CCTGCAGG
TfiI 180 GAATC G^A[AT]TC
1856 GAATC
Tru9I 1166 TTAA T^TAA
1846 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeIII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TseI Tsp45I
Tsp509I TspRI Van91I XcmI XmaCI
XmaI