##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
BalI BamHI BciVI BglII BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BstAPI
BstBI BstEII BstPI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 2732 CTTAAG C^TTAAG
AspI 4502 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1700 TGCGCA TGC^GCA
AvrII 2364 CCTAGG C^CTAGG
BbeI 885 GGCGCC GGCGC^C
BbrPI 1531 CACGTG CAC^GTG
BbvCI 3899 CCTCAGC CCTCAGC(-5/-2)
BcgI 2884 CGAGCAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4297 TGATCA T^GATCA
BfaI 2365 CTAG C^TAG
BfrI 2732 CTTAAG C^TTAAG
BlnI 2364 CCTAGG C^CTAGG
BsaAI 1531 CACGTG [CT]AC^GT[GA]
BsmBI 2540 CGTCTC CGTCTC(1/5)
Bst1107I 502 GTATAC GTA^TAC
BstZ17I 502 GTATAC GTA^TAC
DraIII 3238 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2220 GACAGAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 4297 TGATCA T^GATCA
FseI 2502 GGCCGGCC GGCCGG^CC
FspI 1700 TGCGCA TGC^GCA
KasI 885 GGCGCC G^GCGCC
MaeI 2365 CTAG C^TAG
NarI 885 GGCGCC GG^CGCC
NcoI 2282 CCATGG C^CATGG
NruI 1873 TCGCGA TCG^CGA
PflFI 4502 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1531 CACGTG CAC^GTG
PmlI 1531 CACGTG CAC^GTG
SacI 4014 GAGCTC GAGCT^C
SapI 1913 GCTCTTC GCTCTTC(1/4)
SfoI 885 GGCGCC GGC^GCC
SmaI 2460 CCCGGG CCC^GGG
SphI 2912 GCATGC GCATG^C
StuI 2510 AGGCCT AGG^CCT
Tth111I 4502 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2460 CCCGGG C^CCGGG
XmaI 2460 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 1058 GTAC GT^AC
1999 GTAC
Asp700I 2831 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4420 GAACTCTTTC
BglI 2503 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2961 GCCAGCGGGGC
BlpI 812 GCTGAGC GC^T[ACGT]AGC
3167 GCTAAGC
Bpu1102I 812 GCTGAGC GC^T[ACGT]AGC
3167 GCTAAGC
BsiEI 739 CGACCG CG[GA][CT]^CG
1870 CGGTCG
BsmI 4022 GCATTC GAATGC(1/-1)
4310 GCATTC
BsrDI 4306 CATTGC GCAATG(2/0)
3859 GCAATG
BssHII 1675 GCGCGC G^CGCGC
1693 GCGCGC
CelII 812 GCTGAGC GC^T[ACGT]AGC
3167 GCTAAGC
DraI 4378 TTTAAA TTT^AAA
4644 TTTAAA
EcoNI 2142 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2792 CCTGTTAGAGG
HindIII 3378 AAGCTT A^AGCTT
3667 AAGCTT
RsaI 1058 GTAC GT^AC
1999 GTAC
SfiI 2502 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2960 GGCCAGCGGGGCC
XcmI 2601 CCAGGGGGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3315 CCACAGGTGGGCTGG
XmnI 2831 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4420 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoI XhoII