##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI BamHI BciVI BclI
BglI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI KpnI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2507 GTCGAC GT^[AC][GT]AC
AflII 3531 CTTAAG C^TTAAG
AflIII 3316 ACATGT A^C[GA][CT]GT
AhdI 3591 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3866 GTGCAC G^TGCAC
AocI 636 CCTAAGG CC^T[ACGT]AGG
ApaLI 3866 GTGCAC G^TGCAC
AspEI 3591 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 3531 CTTAAG C^TTAAG
BglII 1268 AGATCT A^GATCT
BlpI 2824 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2824 GCTCAGC GC^T[ACGT]AGC
BsaWI 1004 ACCGGA [AT]^CCGG[AT]
BsiEI 2509 CGACCG CG[GA][CT]^CG
BspLU11I 3316 ACATGT A^CATGT
BsrBI 590 GAGCGG CCGCTC(-3/-3)
BstEII 553 GGTCACC G^GT[ACGT]ACC
BstPI 553 GGTCACC G^GT[ACGT]ACC
Bsu36I 636 CCTAAGG CC^T[ACGT]AGG
CelII 2824 GCTCAGC GC^T[ACGT]AGC
DrdI 1390 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3591 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 636 CCTAAGG CC^T[ACGT]AGG
EcoO65I 553 GGTCACC G^GT[ACGT]ACC
EcoRI 2490 GAATTC G^AATTC
HindIII 815 AAGCTT A^AGCTT
HpaI 3581 GTTAAC GTT^AAC
PshAI 43 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2507 GTCGAC G^TCGAC
SspI 4485 AATATT AAT^ATT
XbaI 3743 TCTAGA T^CTAGA
XcmI 2765 CCACCTTTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AviII 1052 TGCGCA TGC^GCA
1073 TGCGCA
AvrII 2868 CCTAGG C^CTAGG
3551 CCTAGG
BalI 885 TGGCCA TGG^CCA
2859 TGGCCA
BbeI 192 GGCGCC GGCGC^C
1786 GGCGCC
BbrPI 1048 CACGTG CAC^GTG
3819 CACGTG
BbsI 3267 GAAGAC GAAGAC(2/6)
4313 GAAGAC
BcgI 3858 CGAAGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3215 GCAAGTCACTCG
BlnI 2868 CCTAGG C^CTAGG
3551 CCTAGG
BpuAI 3267 GAAGAC GAAGAC(2/6)
4313 GAAGAC
BsaAI 1048 CACGTG [CT]AC^GT[GA]
3819 CACGTG
BsmBI 212 CGTCTC CGTCTC(1/5)
510 GAGACG
BspMI 924 GCAGGT ACCTGC(4/8)
2669 GCAGGT
BssSI 684 CACGAG CACGAG(-5/-1)
2634 CACGAG
BstXI 538 CCAGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1994 CCAGCCTCCTGG
FspI 1052 TGCGCA TGC^GCA
1073 TGCGCA
KasI 192 GGCGCC G^GCGCC
1786 GGCGCC
KspI 1 CCGCGG CCGC^GG
22 CCGCGG
MluNI 885 TGGCCA TGG^CCA
2859 TGGCCA
MscI 885 TGGCCA TGG^CCA
2859 TGGCCA
MslI 921 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3446 CATGGGGATG
NarI 192 GGCGCC GG^CGCC
1786 GGCGCC
NcoI 2147 CCATGG C^CATGG
2360 CCATGG
NruI 107 TCGCGA TCG^CGA
170 TCGCGA
PmaCI 1048 CACGTG CAC^GTG
3819 CACGTG
PmlI 1048 CACGTG CAC^GTG
3819 CACGTG
PstI 2269 CTGCAG CTGCA^G
3702 CTGCAG
SacI 666 GAGCTC GAGCT^C
2133 GAGCTC
SacII 1 CCGCGG CCGC^GG
22 CCGCGG
SfoI 192 GGCGCC GGC^GCC
1786 GGCGCC
StuI 1871 AGGCCT AGG^CCT
2871 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI XhoII XmaCI XmaI