(A) Physical map of pYLTAC7. The map1 shows the locations of recognition sites for endonucleases that cleave the vector once or twice. Abbreviations not defined in text: KmR, kanamycin resistance gene (NPT1); PE. coli, a synthetic E. coli promoter.
(B) Sequence of the cloning site region of the vector upstream of the sacB gene. The indicated primer sequences (R1, R2, R3, L1, L2, and L3) are designed for isolation of end fragments of the inserted DNA by TAIL-PCR. The initiation codon (atg) of sacB is indicated in lowercase letters.