A Database of Human Unidentified Gene-Encoded Large Proteins Analyzed
by Kazusa Human cDNA Project

The HUGE protein database has been created to publicize the fruits of our Human cDNA project at the Kazusa DNA Research Institute. In this project, we plan to sequence and analyze long (>4 kb) human cDNAs and to establish methods by using the sequence data how to predict the primary structure of proteins of various biological activities. Currently, we focus on the analysis of cDNA clones encoding particularly large proteins (>50 kDa). The basic concept underlying our project and the strategies employed have been described elsewhere (Ohara et al., 1997). Our HUGE protein database contains various types of information derived from the predicted primary structure data of newly identified human proteins.

The HUGE protein database are expected to cover various sets of large human proteins of hitherto unidentified functions. They are likely to be involved in cellular structure/motility (such as cytoskeleton, membrane skeleton, and motor proteins), gene expression and nucleic acid metabolism, cell signaling/communication (such as cellular adhesion, signal transduction, channels, and receptors), and so on.

• Kikuno, R., Nagase, T., Nakayama, M. et al., HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE. Nucleic Acids Res. 32, D502-504, (2004).
  
  
• Ohara, O., Nagase, T., Ishikawa, K.-I. et al., Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins. DNA Res., 4, 53-59, (1997).
  
  
• Bibliography

ACKNOWLEDGMENTS

• What's New (2008.9.18)
  
• List of Gene/Protein Characteristic Table
• Description of Gene/Protein CharacteristicTable

• Find genes of interest in HUGE database
  

  Enter KIAA number, accession number, gene symbol or clone name:
  

• To whom who wish to get clones from Kazusa. (cDNA sequences in silico were excluded from HUGE.)
  

  Enter KIAA number, accession number, gene symbol or clone name:
  

• Keyword search
• Homology search

RELATED DATABASES

• HUGEppi
• Mouse DNA microarray

Database for Clone Resources

KOP (Kazusa ORFeome Project) clones Flexi ORF clones and Flexi Halotag ORF clones which include the defined open reading frame are available. Please visit KOP.




OC (ORFeome Collaboration) clones As a member of OC, we started distributing the human ORF clones (OC clones) from the ORFeome collection.

Search All the Human cDNA/ORF Clones

Kazusa Mouse cDNA project

Rouge (ROdent Unidentified Gene-Encoded large proteins) Mouse homologues of KIAA and FLJ cDNA clones. Please visit Rouge.

New site-specific recombination systems

VCre/VloxP and SCre/SloxP system Please visit SSR.

How to obtain Kazusa clone(s) Japan || Othercountries Send a message to office AT kazusa.or.jp