Gene/Protein Characteristic Table for KIAA0025
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Product ID ORK00371
Accession No D14695
Description homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1, transcript variant 1
Clone name ha00594
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (1860 bp)
Predicted protein sequence (422 aa)
Flexi ORF Clone FXC00371
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0025 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 1860 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 591 bp
Genome contig ID gi51511732f_55423565
PolyA signal sequence
(AATAAA,-27)
+----*----+----*----+----*----+----
TTCTGTTCAATAAAGTTTTACTATGAATGACCCTG
Flanking genome sequence
(111731 - 111780)
----+----*----+----*----+----*----+----*----+----*
GCAGAGACTCCTGTCATCCTAGCAGTTTACTCTGCGTTTGTTGTATCTAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 16 f 55523565 55535294 8 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 422 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW82893 2.9e-163 99.8 homocysteine-in...
Homo sapiens
BAF84254 2.1e-151 99.7 unnamed protein...
Homo sapiens
BAD96953 2.1e-151 99.7 homocysteine-in...
Homo sapiens
BAF84777 2.4e-151 99.7 unnamed protein...
Homo sapiens
NP_001010989 1.4e-150 99.7 homocysteine-in...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000626 46 120 PF00240 Ubiquitin
HMMSmart IPR000626 41 118 SM00213 Ubiquitin
ProfileScan IPR000626 41 103 PS50053 Ubiquitin

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 316 SSLSRFLMVMGATVVMYLHHVGW 338 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 16
Experimental conditions
Panel name Stanford G3
Primer_f GGCTTTGACAGGAATGGACT
Primer_r ATTTGTATCACGGCTTCACG
PCR product length 147 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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