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Order Kazusa clone(s) from : |
| Product ID | ORK00502 |
|---|---|
| Accession No | AB002314 |
| Description | FERM and PDZ domain containing 4 |
| Clone name | fg06820 |
| Vector information | |
| cDNA sequence | DNA sequence (6352 bp) Predicted protein sequence (1379 aa) |
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HaloTag ORF Clone |
FHC00502
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| Flexi ORF Clone | FXC00502 |
| Source | Human fetal brain |
| Rouge ID |
mKIAA0316
by Kazusa Mouse cDNA Project
|
| Note | We replaced hg00253, former representative clones for KIAA0316 with fg06820. (2002/12/27) |
Length: 6352 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | Warning |
Integrity of 3' end
| Length of 3'UTR | 1877 bp |
|---|---|
| Genome contig ID | gi89161218f_11966506 |
| PolyA signal sequence (AATAAA,-22) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (683946 - 683995) |
----+----*----+----*----+----*----+----*----+----* |
Length: 1379 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001478 | 144 | 209 | PF00595 | PDZ/DHR/GLGF |
| HMMSmart | IPR001478 | 142 | 212 | SM00228 | PDZ/DHR/GLGF |
| IPR000299 | 259 | 481 | SM00295 | Band 4.1 | |
| ProfileScan | IPR001202 | 90 | 123 | PS50020 | WW/Rsp5/WWP |
| IPR001478 | 135 | 212 | PS50106 | PDZ/DHR/GLGF | |
| IPR000299 | 261 | 576 | PS50057 | Band 4.1 |
RT-PCR
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|---|
Experimental conditions| Primer_f | GATTTTGTCCCAGTACTACGC |
|---|---|
| Primer_r | CAGTAATGTAGACCAGACCAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 13
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GATTTTGTCCCAGTACTACGC |
| Primer_r | CAGTAATGTAGACCAGACCAC |
| PCR product length | 148 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |