KIAA0455

Product ID	ORK00080
Accession No	AB007924
Description	lipid phosphate phosphatase-related protein type 4, transcript variant 1
Clone name	fh02485
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5014 bp) Predicted protein sequence (814 aa)
HaloTag ORF Clone	FHC00080
Flexi ORF Clone	FXC00080
Source	Human fetal brain
Rouge ID	mKIAA0455 by Kazusa Mouse cDNA Project
Note	We replaced hg00513, former representative clones for KIAA0455 with fh02485. (2005/08/06)

Length: 5014 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2568 bp
Genome contig ID	gi89161185f_99402440
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TAAAATCATAGAAATAAAGATTAGATTTCTTCATC
Flanking genome sequence (145286 - 145335)	----+--------+--------+--------+--------+----* AAAATTGGAATAGTTTGGTTTTTACTTCAGAGTCTGAATAGATAATAGCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	99502440	99547724	7	99.2	Perfect prediction

Length: 814 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q7Z2D5	100.0	Lipid phosphate...	Homo sapiens
XP_001489501	98.2	similar to plas...	Equus caballus
AAH42963	99.6	LPPR4 protein [...	Homo sapiens
ACD76642	95.5	plasticity rela...	Felis catus
Q7TME0	95.7	Lipid phosphate...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000326	229	382	PF01569	Phosphoesterase
HMMSmart	IPR000326	230	374	SM00014	Phosphoesterase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	116	TLLPCFYFVELPILASSVVSLYF	138	PRIMARY	23
SOSUI2	2	173	FLMLLSLAFAGPAITIMVGEGIL	195	PRIMARY	23

Primer_f
Primer_r
PCR conditions	°C sec °C sec cycles

Panel name	GeneBridge 4
Primer_f	ATCAGTAAAGCACAGCCTAAC
Primer_r	AGGTGGTATCTCTTCTTAGTG
PCR product length	83 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles