KIAA0906

Product ID	ORK06211
Accession No	AB020713
Description	nucleoporin 210kDa
Clone name	hk10347
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4217 bp) Predicted protein sequence (923 aa)
Source	Human adult brain
Rouge ID	mKIAA0906 by Kazusa Mouse cDNA Project

Length: 4217 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1444 bp
Genome contig ID	gi89161205r_13232737
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- ACTGGTTTATTTAATAAAGTTCTATGTGATTTTTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAGAGGGTGTGATCGTTGCTGAGATTTCTGTCTGATGGTGATGATTTCTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	r	13332737	13359747	20	99.5	Perfect prediction

Length: 923 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10397	99.9	nucleoporin 210...	synthetic construct
BAB84927	99.8	FLJ00172 protei...	Homo sapiens
EAW64151	99.8	nucleoporin 210...	Homo sapiens
Q8TEM1	99.8	Nuclear pore me...	Homo sapiens
EAW64152	99.8	nucleoporin 210...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003343	113	188	PF02368	Bacterial Ig-like
HMMSmart	IPR003343	113	188	SM00635	Bacterial Ig-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	FPAPAKAVVYVSDIQELYIRVVD	23	SECONDARY	23
	2	517	ELSGAMVVGDVLCLATVLTSLEG	539	PRIMARY	23
	3	558	TGVAVARAVGSVTVYYEVAGHLR	580	SECONDARY	23

Primer_f	GAAAATGTCCTTGAGATGGCA
Primer_r	TGATAAGCTGCCATGAAAGTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-