KIAA1253

Product ID	ORK00782
Accession No	AB033079
Description	serine incorporator 1
Clone name	hh14119a
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3109 bp) Predicted protein sequence (472 aa)
HaloTag ORF Clone	FHC00782
Flexi ORF Clone	FXC00782
Source	Human adult brain
Rouge ID	mKIAA1253 by Kazusa Mouse cDNA Project

Length: 3109 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1690 bp
Genome contig ID	gi89161210r_122706198
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- GGCTGTGTTTTACCTCTAAATAAATGAATTCAGAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAGTGTGTAGTGTACTGATTATTTAGCAGCTTAACTTTAGGTGGCCATA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	r	122806198	122834638	10	99.4	Perfect prediction

Length: 472 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
T46332	5.9e-198	100.0	9595 Lambda-PRL...	Arabidopsis tha...
Q9NRX5	2.4e-196	100.0	Serine incorpor...	Homo sapiens
Q5R419	2.8e-196	99.8	Serine incorpor...	Pongo abelii
AAH28607	4.4e-196	99.8	Serine incorpor...	Homo sapiens
AAP97200	8.1e-196	99.8	TDE2 [Homo sapi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005016	34	471	PF03348	TMS membrane protein/tumour differentially expressed protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	SCLHLVCIRCSCDVVEMGSVLGL	26	SECONDARY	23
	2	30	ASWIPCLCGSAPCLLCRCCPSGN	52	SECONDARY	23
	3	62	ALFLLVGVCVACVMLIPGMEEQL	84	SECONDARY	23
	4	103	LVGYKAVYRLCFGLAMFYLLLSL	125	PRIMARY	23
	5	143	GFWFFKFAAAIAIIIGAFFIPEG	165	PRIMARY	23
	6	171	WFYVGMAGAFCFILIQLVLLIDF	193	PRIMARY	23
	7	216	ALLSATALNYLLSLVAIVLFFVY	238	PRIMARY	23
	8	250	AFISVNMLLCVGASVMSILPKIQ	272	SECONDARY	23
	9	278	SGLLQSSVITVYTMYLTWSAMTN	300	SECONDARY	23
	10	332	WHAQGIIGLILFLLCVFYSSIRT	354	PRIMARY	23
	11	403	YSYSFFHFMLFLASLYIMMTLTN	425	SECONDARY	23
	12	447	SSSWIGIVLYVWTLVAPLVLTNR	469	PRIMARY	23

Primer_f	GTCTCCATCTTGTCTGTATCC
Primer_r	TAGCAAACACGGGGCACTTCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-