KIAA1344

Product ID	ORK00808
Accession No	AB037765
Description	thioredoxin domain containing 16, transcript variant 1
Clone name	fj00476s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4552 bp) Predicted protein sequence (858 aa)
HaloTag ORF Clone	FHC00808
Flexi ORF Clone	FXC00808
Source	Human fetal brain
Rouge ID	mKIAA1344 by Kazusa Mouse cDNA Project
Note	We replaced fj00476, former representative clones for KIAA1344 with fj00476s1. (2002/5/10)

Length: 4552 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1713 bp
Genome contig ID	gi51511730r_51867059
PolyA signal sequence (AATAAA,-30)	+--------+--------+----*----+---- TTTTAAATAAAGTGTCTGGGTTTCAGAATACCTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACAAGCCTCCTTTAGTCTCCACTGTGCCCTCTCGGACCACTCTCCCTCTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	r	51967059	52088963	21	99.4	Perfect prediction

Length: 858 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI50206	99.9	Thioredoxin dom...	Homo sapiens
AAI44470	99.2	Unknown (protei...	Homo sapiens
XP_001103706	95.2	similar to Prot...	Macaca mulatta
XP_537446	87.1	similar to Prot...	Canis lupus fam...
EDL20686	75.6	RIKEN cDNA 5730...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013766	425	528	PF00085	Thioredoxin domain

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	31	QLIMFSGFNVFRVGISFVIMCIF	53	PRIMARY	23
SOSUI2	2	151	TLFDVNAIVAHVLFALLFSEVKY	173	PRIMARY	23

Primer_f	CTTGTTTTGGTGAATCTGCAT
Primer_r	AATCATAAGCTGGAAGAGGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-