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Order Kazusa clone(s) from : |
| Product ID | ORK00817 |
|---|---|
| Accession No | AB037790 |
| Description | eukaryotic translation initiation factor 2-alpha kinase 1, transcript variant 1 |
| Clone name | fj03222 |
| Vector information | |
| cDNA sequence | DNA sequence (4391 bp) Predicted protein sequence (653 aa) |
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HaloTag ORF Clone |
FHC00817
|
| Flexi ORF Clone | FXC00817 |
| Source | Human fetal brain |
| Rouge ID |
mKIAA1369
by Kazusa Mouse cDNA Project
|
Length: 4391 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 2427 bp |
|---|---|
| Genome contig ID | gi89161213r_5928624 |
| PolyA signal sequence (AATAAA,-28) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (99782 - 99733) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 7 | r | 6028406 | 6065311 | 15 | 99.4 | Perfect prediction |
Length: 653 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR000719 | 190 | 263 | PD000001 | Protein kinase |
| IPR000719 | 442 | 597 | PD000001 | Protein kinase | |
| HMMPfam | IPR000719 | 190 | 606 | PF00069 | Protein kinase |
| HMMSmart | IPR001245 | 190 | 606 | SM00219 | Tyrosine protein kinase |
| IPR002290 | 190 | 606 | SM00220 | Serine/threonine protein kinase | |
| ProfileScan | IPR000719 | 190 | 606 | PS50011 | Protein kinase |
| ScanRegExp | IPR000719 | 196 | 220 | PS00107 | Protein kinase |
| IPR008271 | 461 | 473 | PS00108 | Serine/threonine protein kinase |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | ATCTTTGCGTTGCTTCATCTC |
|---|---|
| Primer_r | ATGGTTTTGATAAGAGGCAGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 7
Experimental conditions| Panel name | unigene |
|---|---|
| Primer_f | - |
| Primer_r | - |
| PCR product length | - |
| PCR conditions | - |