KIAA1560

Product ID	ORK05326
Accession No	AB046780
Description	glycerol-3-phosphate acyltransferase, mitochondrial
Clone name	fh19959
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5674 bp) Predicted protein sequence (662 aa)
Source	Human fetal brain
Rouge ID	mKIAA1560 by Kazusa Mouse cDNA Project

Length: 5674 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3685 bp
Genome contig ID	gi89161187r_113799614
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGTAGTTTTGTTTTAATAAACCAAAAATTGAACAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTCTTGATGTGTTTGATTTGTATTTAAAACAGTTTCCTACATAATTCAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	r	113899614	113923508	16	99.3	Perfect prediction

Length: 662 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH30783	100.0	Glycerol-3-phos...	Homo sapiens
BAD18772	99.8	unnamed protein...	Homo sapiens
XP_001145374	99.8	mitochondrial g...	Pan troglodytes
CAD89932	99.7	hypothetical pr...	Homo sapiens
CAD89967	99.7	hypothetical pr...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002123	39	189	PF01553	Phospholipid/glycerol acyltransferase
HMMSmart	IPR002123	58	191	SM00563	Phospholipid/glycerol acyltransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	QEMVATVSPAMIRLTGWVLLKLF	31	SECONDARY	23
SOSUI2	2	408	NGVLHVFIMEAIIACSLYAVLN	429	PRIMARY	22

Primer_f	GGAGCACCAGCAGTTTATCAC
Primer_r	TGTGGCACTCTCAGCATATAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-