FLJ00009

Product ID	ORK05033
Accession No	AK024420
Clone name	as00009
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4362 bp) Predicted protein sequence (540 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 540 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB15710	7.8e-205	100.0	FLJ00009 protei...	Homo sapiens
EAW76479	5.5e-192	99.8	solute carrier ...	Homo sapiens
BAB15784	5.7e-192	99.8	FLJ00100 protei...	Homo sapiens
BAB15711	6.3e-192	99.8	FLJ00010 protei...	Homo sapiens
Q9BXP2	7.2e-192	99.8	Solute carrier ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK024494	5.4e-154	99.8	FLJ00100
AK024421	5.9e-154	99.8	FLJ00010
AK090458	2.2e-142	98.1	FLJ00379
AK024466	1.6e-141	100.0	FLJ00059
AB033002	2.7e-15	28.6	KIAA1176

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	12	TGILGVHWHFGTTARLLALAQLV	34	PRIMARY	23
	2	45	AVVTVFYLVAYAAVDLSCLSLEW	67	PRIMARY	23
	3	91	SCLLMMFLISPGAAGGSLLLMGL	113	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test