KIAA1026

Gene/Protein Characteristic Table for KIAA1026

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Product ID	ORK00716
Accession No	AB028949
Description	kazrin, periplakin interacting protein
Clone name	fh00114
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5592 bp) Predicted protein sequence (519 aa)
Source	Human fetal brain
Rouge ID	mKIAA1026 by Kazusa Mouse cDNA Project

Features of the cloned cDNA sequence

Description

Length: 5592 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	4032 bp
Genome contig ID	gi89161185f_14697865
PolyA signal sequence (None)	+--------+--------+----*----+---- AGTTTTTAAATTGCATTGCCGTTTCTTTCTTTATG
Flanking genome sequence (567780 - 567829)	----+--------+--------+--------+--------+----* AAAAAAAAGAAAAAAAGAAAATTGTTTCATTTAATTTATTTGCACAAATG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	14797787	15265643	7	99.4	Perfect prediction

Features of the protein sequence

Description

Length: 519 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG09940	1.1e-125	100.0	kazrin protein ...	synthetic construct
AAS86434	3.6e-100	97.3	kazrin isoform ...	Homo sapiens
Q674X7	5.5e-100	97.3	Kazrin.	Homo sapiens
Q5FWS6	5e-94	92.2	Kazrin.	Rattus norvegicus
NP_001103154	1.6e-93	92.2	kazrin isoform ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Expression profile

Description

RT-PCR-ELISA

Experimental conditions

Primer_f	GCCCCATCAATAAGCCATCTG
Primer_r	ATAGGGTTGTGCAGGTTCTTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 1

Experimental conditions

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-

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