KIAA2025

Product ID	ORK06615
Accession No	AB095945
Description	ring finger and CCCH-type domains 1
Clone name	fh08764
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone fh08764 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCGG ACGCGTGGG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (5273 bp) Predicted protein sequence (1109 aa)
Source	Human fetal brain
Rouge ID	mKIAA2025 by Kazusa Mouse cDNA Project

Length: 5273 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1943 bp
Genome contig ID	gi89161185r_172072547
PolyA signal sequence (None)	+--------+--------+----*----+---- TTTCCAGTTTTCTTATACATTTGTCTTCTTTTTTT
Flanking genome sequence (99993 - 99944)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATATGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	172172540	172228674	19	99.6	Perfect prediction

Length: 1109 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q5TC82	100.0	Roquin; RING fi...	Homo sapiens
XP_001151649	99.8	hypothetical pr...	Pan troglodytes
XP_001102746	99.0	roquin isoform ...	Macaca mulatta
CAH70709	100.0	ring finger and...	Homo sapiens
XP_537186	96.9	similar to roqu...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000571	390	416	PF00642	Zinc finger
HMMSmart	IPR000571	389	416	SM00356	Zinc finger
ScanRegExp	IPR001841	9	18	PS00518	Zinc finger

Primer_f	CCCTATACCCATTGAGATTCC
Primer_r	CTGAGGTACGAAGGTCTGATC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-