KIAA1690

Product ID	ORK07133
Accession No	AB051477
Description	transmembrane protein 108
Clone name	fh27434
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4835 bp) Predicted protein sequence (482 aa)
Source	Human fetal brain
Rouge ID	mKIAA1690 by Kazusa Mouse cDNA Project

Length: 4835 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3245 bp
Genome contig ID	gi89161205f_134226607
PolyA signal sequence (None)	+--------+--------+----*----+---- GCACTCCAGCCTGGGCAACGGAGCTAGACTCCATC
Flanking genome sequence (364612 - 364661)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAGAAAAAAAAAAACCATAGACTTTGGAGTCCAGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	f	134430790	134591217	3	98.9	Terminal No-hit

Length: 482 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAQ88747	4.8e-120	99.8	KRSL1875 [Homo ...	Homo sapiens
Q6UXF1	5.2e-120	99.8	Transmembrane p...	Homo sapiens
XP_001115415	9.6e-112	93.5	similar to muci...	Macaca mulatta
XP_516759	1.1e-93	91.8	similar to Tran...	Pan troglodytes
CAB66513	8.6e-93	100.0	hypothetical pr...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ScanRegExp	IPR010916	1	37	PS00430	TonB box

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	YCQLLSFLLILALTEALAFAIQE	25	PRIMARY	23
SOSUI2	2	455	TICLSKMDIAWVILAISVPISSC	477	PRIMARY	23

Primer_f	TTGGCTTCAGCTCTCCTCATC
Primer_r	CACACTGCAGAAGGGTAACAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	CCR
Primer_f	TTGGCTTCAGCTCTCCTCATC
Primer_r	CACACTGCAGAAGGGTAACAC
PCR product length	136 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles