KIAA1382

Product ID	ORK00818
Accession No	AB037803
Description	solute carrier family 38, member 2, transcript variant 1
Clone name	fj05641s1
Vector information	The cDNA fragment was originally inserted at SalI-NotI site ... The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a RT-PCR product (1-289) was subcloned upstream of the cDNA (290-4444). The adjacent sequence to the 5' end of the cDNA is as follows: GGGCCCCCCC TCGAGGTCGA CCCACGCGT-5' end of cDNA.
cDNA sequence	DNA sequence (4444 bp) Predicted protein sequence (506 aa)
HaloTag ORF Clone	FHC00818
Flexi ORF Clone	FXC00818
Source	Human fetal brain
Rouge ID	mKIAA1382 by Kazusa Mouse cDNA Project
Note	We replaced fj05641, former representative clones for KIAA1382 with fj05641s1. (2002/5/10)

Length: 4444 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2923 bp
Genome contig ID	gi89161190r_44938239
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- TTAACTGAACTAAAATAAATACATGCTTAATCCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTAGCAGTGTTTTTTGTTTTGTTTTGTTTTGTTTTTTTCAGGTAATGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	45038239	45051343	15	99.3	Perfect prediction

Length: 506 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAF84333	2.7e-196	99.8	unnamed protein...	Homo sapiens
A1YG32	3.1e-196	99.8	Sodium-coupled ...	Pan paniscus
CAC51434	4.1e-196	99.8	putative 40-9-1...	Homo sapiens
XP_001095418	2.3e-192	96.8	similar to solu...	Macaca mulatta
XP_543722	2e-183	92.5	similar to solu...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013057	69	495	PF01490	Amino acid transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	72	SFGMSVFNLSNAIVGSGILGLSY	94	SECONDARY	23
	2	101	IALFIILLTFVSIFSLYSVHLLL	123	PRIMARY	23
	3	161	AMSSYLFIVKYELPLVIQALTNI	183	SECONDARY	23
	4	192	LNGNYLVLLVSLVVILPLSLFRN	214	PRIMARY	23
	5	217	YLGYTSGLSLLCMVFFLIVVIC	238	PRIMARY	22
	6	293	VYAVPILIFSFVCHPAVLPIYEE	315	PRIMARY	23
	7	327	VSKISFFAMFLMYLLAALFGYLT	349	PRIMARY	23
	8	360	HTYSSILGTDILLLIVRLAVLMA	382	PRIMARY	23
	9	386	TVPVVIFPIRSSVTHLLCASKD	407	SECONDARY	22
	10	411	WRHSLITVSILAFTNLLVIFVPT	433	PRIMARY	23
	11	439	GFIGASAASMLIFILPSAFYIKL	461	PRIMARY	23
	12	474	GALFFLLSGVLVMTGSMALIVLD	496	PRIMARY	23

Primer_f	TTCCGTGGATGTTTCAGTTAC
Primer_r	AACCCAAGACCCAAACCTGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-