KIAA1616

Product ID	ORK07220
Accession No	AB046836
Description	transient receptor potential cation channel, subfamily M, member 3
Clone name	fj13825
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3957 bp) Predicted protein sequence (1017 aa)
Source	Human fetal brain
Rouge ID	mKIAA1616 by Kazusa Mouse cDNA Project

Length: 3957 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	901 bp
Genome contig ID	gi89161216r_72239788
PolyA signal sequence (None)	+--------+--------+----*----+---- ATGAATTCATTCACTGAATATGTAATATTGAACTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAACAGTGGACAGAACTAGCCTGAAATTGCCATG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	72339788	72424836	11	99.4	Perfect prediction

Length: 1017 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW62509	99.9	transient recep...	Homo sapiens
EAW62506	99.9	transient recep...	Homo sapiens
CAM13090	99.9	transient recep...	Homo sapiens
EAW62511	99.9	transient recep...	Homo sapiens
EAW62507	99.9	transient recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005821	214	422	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	183	FWFYTLAYIGYLMLFNYIVLVKM	205	PRIMARY	23
	2	250	YWNVTDLIAILLFSVGMILRLQD	272	PRIMARY	23
	3	279	GRVIYCVNIIYWYIRLLDIFGVN	301	SECONDARY	23
	4	312	GKMMIDMMYFVIIMLVVLMSFGV	334	PRIMARY	23
	5	403	MACYLLVANILLVNLLIAVFNNT	425	PRIMARY	23

Primer_f	CCACAGAAGGCTCAAGAATCC
Primer_r	GCCTTAAATCCCCTGTGTCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	CCR
Primer_f	CCACAGAAGGCTCAAGAATCC
Primer_r	GCCTTAAATCCCCTGTGTCTG
PCR product length	198 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles