KIAA0069

Product ID	ORK00394
Accession No	D31885
Description	ADP-ribosylation factor-like 6 interacting protein 1
Clone name	ha01508
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2272 bp) Predicted protein sequence (226 aa)
HaloTag ORF Clone	FHC00394
Flexi ORF Clone	FXC00394
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0069 by Kazusa Mouse cDNA Project

Length: 2272 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1591 bp
Genome contig ID	gi51511732r_18610517
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- GGAAAAAATAAAAAAAGCTGGAATCTTCATGTCTC
Flanking genome sequence (99967 - 99918)	----+--------+--------+--------+--------+----* ACTTTCTTTATAACTTAGCCAATATAATATTTATGATTGGATTTATGAAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	r	18710484	18720358	6	99.2	Perfect prediction

Length: 226 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15041	8.4e-89	100.0	ADP-ribosylatio...	Homo sapiens
XP_001082004	3.2e-88	99.5	ADP-ribosylatio...	Macaca mulatta
Q5R454	5.3e-88	99.0	ADP-ribosylatio...	Pongo abelii
BAF83826	1.7e-87	99.5	unnamed protein...	Homo sapiens
XP_537908	1.7e-87	98.5	similar to ARL-...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	42	214	PD352110	NULL

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	VRVSVGGLVGEVACACRDCIPET	23	SECONDARY	23
	2	65	AWFPPAIMGVVSLVFLIIYYLDP	87	PRIMARY	23
	3	93	VSCFVMFLCLADYLVPILAPRIF	115	PRIMARY	23
	4	160	YFMTMIVSLAAVAWVGQQVHNLL	182	PRIMARY	23
	5	184	TYLIVTSLLLLPGLNQHGIILKY	206	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	GCAGACTTGAGGTGATGATAG
Primer_r	CAATGGGCACCACTGTTCACA
PCR product length	181 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles