KIAA0195

Product ID	ORK00451
Accession No	D83779
Description	KIAA0195
Clone name	ha02752
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5022 bp) Predicted protein sequence (1410 aa)
HaloTag ORF Clone	FHC00451
Flexi ORF Clone	FXC00451
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0195 by Kazusa Mouse cDNA Project

Length: 5022 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	748 bp
Genome contig ID	gi51511734f_70864331
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- TGTAGACTGGTTTGTATTAAAATGTGTCAATTGCT
Flanking genome sequence (143429 - 143478)	----+--------+--------+--------+--------+----* AAGAAATACCTGTGGCTGGTCTGTAAGGCCACTCCAGGGTCTGCCCACCC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	17	f	70964331	71007758	32	99.9	Perfect prediction

Length: 1410 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001096655	99.3	hypothetical pr...	Macaca mulatta
BAG57737	99.1	unnamed protein...	Homo sapiens
BAH14431	99.0	unnamed protein...	Homo sapiens
XP_001095555	98.6	hypothetical pr...	Macaca mulatta
EDL34520	95.3	RIKEN cDNA 2310...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR008250	223	247	PF00122	E1-E2 ATPase-associated region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	27	TGVCTCWGGAFLSGVTTFIWACL	49	SECONDARY	23
	2	119	FHWPGASLMLLAVLLLLGCCGGQ	141	PRIMARY	23
	3	147	GVGLVNASALFLLLLLNLVLIGR	169	PRIMARY	23
	4	337	YAVPVVLAGFLITNALRFIFSAP	359	PRIMARY	23
	5	372	QVNGVLPILPLLFPVLWVLATAC	394	PRIMARY	23
	6	1146	CFLFLLQCQLTLVVIQFLSCLVQ	1168	PRIMARY	23
	7	1175	TTDILWLSCFCYPLLSISLLGKP	1197	PRIMARY	23
	8	1223	LLCFLLKFSLTISSCLICFGFTL	1245	SECONDARY	23
	9	1287	QKLTAALIVLHTVFISITHVHR	1308	SECONDARY	22
	10	1317	PLTNLWWAVTVPVVLLGQVVQTA	1339	PRIMARY	23
	11	1359	VPLLTWLLGCLSLVLVVVTNEIV	1381	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	GTCTCCCGCCTGAACCTGAAG
Primer_r	AGATTCCCCAAGACTGACAGG
PCR product length	102 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles