KIAA0255

Product ID	ORK07128
Accession No	D87444
Description	transmembrane 9 superfamily protein member 4
Clone name	ha07076
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4028 bp) Predicted protein sequence (692 aa)
HaloTag ORF Clone	FHC07128
Flexi ORF Clone	FXC07128
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0255 by Kazusa Mouse cDNA Project

Length: 4028 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1810 bp
Genome contig ID	gi51511747f_30061200
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- TTTGGTCGGATTGTAAATAAATATACCATTGTCCT
Flanking genome sequence (157520 - 157569)	----+--------+--------+--------+--------+----* ACTGTTTTGTCTTGAATCTCATGCAGGGGTTGGAGGTCTGAGGTGAGACA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	f	30161200	30218718	18	99.5	Perfect prediction

Length: 692 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH22850	100.0	TM9SF4 protein ...	Homo sapiens
Q92544	100.0	Transmembrane 9...	Homo sapiens
Q5RDY2	99.8	Transmembrane 9...	Pongo abelii
XP_001108787	99.7	transmembrane 9...	Macaca mulatta
A5D7E2	98.7	Transmembrane 9...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004240	104	650	PF02990	Nonaspanin (TM9SF)

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	332	SIINSVVVVFFLSGILSMIIIRT	354	PRIMARY	23
	2	395	SSLLGSGIQLFCMILIVIFVAML	417	PRIMARY	23
	3	428	LMTTACFLFMFMGVFGGFSAGRL	450	SECONDARY	23
	4	467	ATLYPGVVFGICFVLNCFIWGK	488	PRIMARY	22
	5	496	FPTMVALLCMWFGISLPLVYLGY	518	PRIMARY	23
	6	551	VGILMAGILPFGAMFIELFFIFS	573	PRIMARY	23
	7	586	FLFLVFIILVVSCSQISIVMVYF	608	PRIMARY	23
	8	621	NFLVSGGSAFYVLVYAIFYFVNK	643	PRIMARY	23
	9	657	GYTALMVLSFWLLTGTIGFYAAY	679	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	GGGAACCCAAGCAGTAGAGAG
Primer_r	CTTTATGAGGTGTCCGTTTCC
PCR product length	118 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles