KIAA1244

Product ID	ORK00777
Accession No	AB033070
Description	ARFGEF family member 3
Clone name	hf00438s1
Vector information	The cDNA fragment was originally inserted at SalI-NotI site ... The cDNA fragment was originally inserted at SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR) and a cDNA fragment (1-636) was subcloned upstream of the cDNA (637-7932). The adjacent sequence to the 5' end of the cDNA is as follows: CTCGAGCAGC TGAAGCTTTC ACAAGTTTGT ACAAAAAAGC AGGCTCTTC-5' end of cDNA.
cDNA sequence	DNA sequence (7932 bp) Predicted protein sequence (1872 aa)
HaloTag ORF Clone	FHC00777
Flexi ORF Clone	FXC00777
Source	Human adult brain
Rouge ID	mKIAA1244 by Kazusa Mouse cDNA Project
Note	We replaced hf00438, former representative clones for KIAA1244 with hf00438s1. (2002/5/10)

Length: 7932 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2313 bp
Genome contig ID	gi89161210f_138518411
PolyA signal sequence (None)	+--------+--------+----*----+---- ACTCCAGACTGGGTGACAAGAGTGAAACTCCATCT
Flanking genome sequence (183220 - 183269)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAGTGAATACTGTATCCCAAAGTATGTTAGTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	f	138618411	138701629	25	100.0	Perfect prediction

Length: 1872 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_518767	99.8	hypothetical pr...	Pan troglodytes
XP_001095348	98.9	similar to CG59...	Macaca mulatta
BAG10007	100.0	brefeldin A-inh...	synthetic construct
AAL04174	100.0	BIG3 [Homo sapi...	Homo sapiens
Q3UGY8	93.0	Brefeldin A-inh...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR015403	938	1014	PF09324	Protein of unknown function DUF1981
HMMSmart	IPR000904	281	493	SM00222	SEC7-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	TAPALSGPVARTIYYIAAELVRL	27	SECONDARY	23
SOSUI2	2	114	GIEACYAAVSCVCTLLGALDELS	136	PRIMARY	23

Primer_f	TTGAGGAGCTGAAGGATGGGG
Primer_r	TGTCAAGGCTCCTCTCGTTCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TTGAGGAGCTGAAGGATGGGG
Primer_r	TGTCAAGGCTCCTCTCGTTCC
PCR product length	135 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles