KIAA1126

Product ID	ORK06877
Accession No	AB032952
Description	solute carrier family 45, member 4
Clone name	hg02277
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6873 bp) Predicted protein sequence (618 aa)
Source	Human adult brain
Rouge ID	mKIAA1126 by Kazusa Mouse cDNA Project

Length: 6873 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	5015 bp
Genome contig ID	gi51511724r_142186456
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TCCTCTGTGGCTGACTCAATAAACTTTTCCCTCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACATGATGATGTGGGTGTGTCTGTCACTTCCCTGTGGCCTCCTGCCCTCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	r	142286456	142299091	5	99.0	Perfect prediction

Length: 618 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI57841	100.0	SLC45A4 protein...	Homo sapiens
EAW92223	99.8	hCG1729650, iso...	Homo sapiens
AAH90891	99.8	SLC45A4 protein...	Homo sapiens
Q5BKX6	99.8	Solute carrier ...	Homo sapiens
EAW92224	99.8	hCG1729650, iso...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	RQPIGIVLTVLGVVVLDFSADAT	25	SECONDARY	23
	2	44	MALNIHAFSAGLGGAIGYVLGGL	66	SECONDARY	23
	3	82	QVLFFFAAIIFTVSVALHLFSID	104	PRIMARY	23
	4	357	ELMRLCLCHLLTWFSVIAEAVFY	379	PRIMARY	23
	5	409	KMGCWGLVIYAATGAICSALLQK	431	SECONDARY	23
	6	437	DLSVRVIYVLGTLGFSVGTAVMA	459	PRIMARY	23
	7	465	YVAMVTISTMGIVSMSISYCPYA	487	PRIMARY	23
	8	513	DCAILSCQVYISQILVASALGGV	535	PRIMARY	23
	9	545	IPMVASVGSFLGFLTATFLVIYP	567	PRIMARY	23

Primer_f	CAGGAGTGTTTCTTGAATGCC
Primer_r	AGCCATATAAGGGAACAGTTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-