KIAA0374

Product ID	ORK01972
Accession No	AB002372
Description	syntaphilin
Clone name	hh00327
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5530 bp) Predicted protein sequence (584 aa)
HaloTag ORF Clone	FHC01972
Flexi ORF Clone	FXC01972
Source	Human adult brain
Rouge ID	mKIAA0374 by Kazusa Mouse cDNA Project

Length: 5530 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3271 bp
Genome contig ID	gi51511747f_1094999
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- GCAGACAACTCTGAATTAAAGCATGAAAACACAGC
Flanking genome sequence (142972 - 143021)	----+--------+--------+--------+--------+----* AAGCCCCTTGGCCTGTTTTCTTTATACTGGGTGGTTTCTCTAAGGGGAGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	f	1194999	1237969	7	99.2	Perfect prediction

Length: 584 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O15079	3e-177	100.0	Syntaphilin.	Homo sapiens
XP_001153223	9.8e-177	99.8	syntaphilin iso...	Pan troglodytes
XP_001112820	2e-174	98.5	syntaphilin [Ma...	Macaca mulatta
XP_001497716	1.6e-163	93.7	similar to Synt...	Equus caballus
AAH35788	7.7e-161	100.0	Syntaphilin [Ho...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040905	1.8e-23	41.8	KIAA1472

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	10	WVVGTCAPALFNSLVEAAVVCQA	32	PRIMARY	23
SOSUI2	2	514	HYIVDLLAVVVPAVPTVAWLCR	535	PRIMARY	22

Primer_f	GGATGGCCTAATATGGGAATG
Primer_r	TCTTACAGCACTTTGGGGAGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GGATGGCCTAATATGGGAATG
Primer_r	TCTTACAGCACTTTGGGGAGG
PCR product length	151 bp
PCR conditions	95 °C15 sec64 °C60 sec32 cycles