KIAA1165

Product ID	ORK00753
Accession No	AB032991
Description	Nedd4 family interacting protein 2
Clone name	hk01631
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4415 bp) Predicted protein sequence (284 aa)
HaloTag ORF Clone	FHC00753
Flexi ORF Clone	FXC00753
Source	Human adult brain
Rouge ID	mKIAA1165 by Kazusa Mouse cDNA Project

Length: 4415 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3560 bp
Genome contig ID	gi51511729f_78853496
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- AATTTTAATAAACAAAATATCATCTTTTTGAAAAT
Flanking genome sequence (174717 - 174766)	----+--------+--------+--------+--------+----* AATTTATGTTCATTTCTACTTTGTAAAGTATCATTGGTCACCTACGTCAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	13	f	78953496	79028211	8	99.2	Perfect prediction

Length: 284 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC77372	1.7e-100	100.0	putative NFkB a...	Homo sapiens
Q9NV92	3.2e-100	99.6	NEDD4 family-in...	Homo sapiens
CAH93486	5.2e-99	98.9	hypothetical pr...	Pongo abelii
XP_522688	7.4e-99	98.9	similar to puta...	Pan troglodytes
XP_001092556	9.5e-97	97.2	Nedd4 family in...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	179	GIFMLAFFMAFIFNWLGFCLSFC	201	PRIMARY	23
	2	210	YGAICGFGLSLIKWILIVRFSDY	232	PRIMARY	23
	3	239	GQYWLWWIFLVLGLLLFFRGFVN	261	PRIMARY	23

Primer_f	AGATGTGGAAGTGTGAACCTG
Primer_r	GCAACCCCAACCTAATTTCAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AGATGTGGAAGTGTGAACCTG
Primer_r	GCAACCCCAACCTAATTTCAC
PCR product length	152 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles