KIAA0879

Product ID	ORK00142
Accession No	AB020686
Description	ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative)
Clone name	hk07371
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4312 bp) Predicted protein sequence (461 aa)
HaloTag ORF Clone	FHC00142
Flexi ORF Clone	FXC00142
Source	Human adult brain
Rouge ID	mKIAA0879 by Kazusa Mouse cDNA Project

Length: 4312 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2902 bp
Genome contig ID	gi89161210f_46115246
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- AATAGTGTGTAAATAAAATAACATCAGTGTGCTTT
Flanking genome sequence (106993 - 107042)	----+--------+--------+--------+--------+----* AAAGAAATGTGTATGTAGTGCCTTAATTTAAATTAAAATATTTTTGACTG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	f	46205871	46222237	4	99.2	Perfect prediction

Length: 461 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9Y6X5	2.2e-200	100.0	Ectonucleotide ...	Homo sapiens
XP_518515	4e-200	99.8	ectonucleotide ...	Pan troglodytes
EAX04292	8.8e-200	99.8	ectonucleotide ...	Homo sapiens
BAF83965	3.6e-199	99.6	unnamed protein...	Homo sapiens
Q5RAC0	4.6e-196	98.2	Ectonucleotide ...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002591	34	347	PF01663	Type I phosphodiesterase/nucleotide pyrophosphatase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	SFNVFIMKLLVILLFSGLITGFR	25	PRIMARY	23
SOSUI2	2	412	LPEAIAIVIGSLLVLTMLTCLII	434	PRIMARY	23

Primer_f	TTGGGTGTCTCCTTCTTGTGC
Primer_r	TACACATTTTCCATCTCCTCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TTGGGTGTCTCCTTCTTGTGC
Primer_r	TACACATTTTCCATCTCCTCC
PCR product length	139 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles