FLJ00350

Product ID	ORK06359
Accession No	AK090433
Clone name	sg00019
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6655 bp) Predicted protein sequence (601 aa)
Source	Human spleen
Rouge ID	mFLJ00350 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 601 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC03414	0	100.0	FLJ00350 protei...	Homo sapiens
BAB84890	2.7e-201	99.6	FLJ00135 protei...	Homo sapiens
AAH29271	6.2e-199	99.6	PIGO protein [H...	Homo sapiens
Q8TEQ8	6.7e-199	99.6	GPI ethanolamin...	Homo sapiens
XP_001165467	3.2e-198	99.2	phosphatidylino...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK074064	4.1e-218	99.6	FLJ00135

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	82	RMAGGTALLAASCFICLLASQWA	104	PRIMARY	23
	2	108	GFPFCPLLLTPVAWGLVGAIAYA	130	PRIMARY	23
	3	135	TIELKLDLVLLGAVAAVSSFLPF	157	PRIMARY	23
	4	173	TLFPIPGPVLLLLLFRLAVFFSD	195	PRIMARY	23
	5	203	RATPFLLGSFILLLVVQLHWEGQ	225	PRIMARY	23
	6	251	AYALRLGIGLLLCTRLAGLFHRC	273	SECONDARY	23
	7	299	NLWYGACVAALVALLAAVRLWL	320	PRIMARY	22
	8	332	PMLFVRWGLPLMALGTAAYWALA	354	SECONDARY	23
	9	372	SMVLPRAVAGLAASGLALLLWKP	394	SECONDARY	23
	10	456	AAYQLGSVYSAAMVTALTLLAFP	478	PRIMARY	23
	11	490	VFLLLFLQSFLLLHLLAAGIPVT	512	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test