FLJ00316

Product ID	ORK05282
Accession No	AK131094
Clone name	sh06964
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5431 bp) Predicted protein sequence (649 aa)
HaloTag ORF Clone	FHC05282
Flexi ORF Clone	FXC05282
Source	Human spleen
Rouge ID	mFLJ00316 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 649 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC85144	5e-211	100.0	FLJ00316 protei...	Homo sapiens
AAL02054	4.7e-208	100.0	guanylate bindi...	Homo sapiens
Q96PP9	1.7e-206	99.1	Guanylate-bindi...	Homo sapiens
BAG35327	5.1e-206	98.8	unnamed protein...	Homo sapiens
AAH70055	5.1e-206	98.8	Guanylate bindi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK090479	1.4e-52	57.1	FLJ00401

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003191	42	305	PF02263	Guanylate-binding protein
HMMPfam	IPR003191	307	603	PF02841	Guanylate-binding protein

Primer_f
Primer_r
PCR conditions	test