FLJ00385

Product ID	ORK05184
Accession No	AK090464
Clone name	sj03289
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4240 bp) Predicted protein sequence (509 aa)
Source	Human spleen
Rouge ID	mFLJ00385 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Length: 509 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC03445	1.1e-178	100.0	FLJ00385 protei...	Homo sapiens
BAA11363	1.6e-153	99.8	membrane-bound-...	Homo sapiens
XP_001168435	4.1e-133	94.8	similar to FLJ0...	Pan troglodytes
CAC10229	6.8e-130	100.0	immunoglobulin ...	Homo sapiens
AAO17822	7.4e-130	99.7	anti-rabies SOJ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK090461	7.4e-07	24.5	FLJ00382

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	NULL	76	158	PF07654	NULL
	IPR007110	83	148	PF00047	Immunoglobulin-like
	NULL	237	326	PF07654	NULL
	IPR007110	247	316	PF00047	Immunoglobulin-like
	NULL	345	431	PF07654	NULL
	IPR007110	353	420	PF00047	Immunoglobulin-like
HMMSmart	IPR003597	85	156	SM00407	Immunoglobulin C-type
	IPR003597	249	324	SM00407	Immunoglobulin C-type
	IPR003597	355	428	SM00407	Immunoglobulin C-type
ProfileScan	IPR007110	69	162	PS50835	Immunoglobulin-like
	NULL	174	222	PS50311	NULL
	IPR000694	175	240	PS50099	Proline-rich region
	IPR007110	231	330	PS50835	Immunoglobulin-like
	IPR007110	339	435	PS50835	Immunoglobulin-like
ScanRegExp	IPR003006	144	150	PS00290	Immunoglobulin/major histocompatibility complex
ScanRegExp	IPR003006	416	422	PS00290	Immunoglobulin/major histocompatibility complex

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	456	GLWTTITIFITLFLLSVCYSATV	478	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test