##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AclI AgeI Aor51HI AscI AviII
BciVI BclI Bsp1407I BspDI BsrGI
BssHII ClaI Eco47III EcoT22I FbaI
FseI FspI HindIII HpaI KspI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI Psp1406I PvuI SacII
SalI SfiI SnaBI SpeI SphI
SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 6119 GGTACC G^GTACC
Alw44I 654 GTGCAC G^TGCAC
ApaLI 654 GTGCAC G^TGCAC
AseI 8405 ATTAAT AT^TAAT
AsnI 8405 ATTAAT AT^TAAT
Asp718I 6119 GGTACC G^GTACC
BsiWI 232 CGTACG C^GTACG
BspLU11I 1822 ACATGT A^CATGT
Bst1107I 8593 GTATAC GTA^TAC
BstEII 4307 GGTAACC G^GT[ACGT]ACC
BstPI 4307 GGTAACC G^GT[ACGT]ACC
BstZ17I 8593 GTATAC GTA^TAC
CpoI 2254 CGGACCG CG^G[AT]CCG
EcoO65I 4307 GGTAACC G^GT[ACGT]ACC
EcoRI 6758 GAATTC G^AATTC
EcoRV 7132 GATATC GAT^ATC
KpnI 6119 GGTACC GGTAC^C
MfeI 7882 CAATTG C^AATTG
MunI 7882 CAATTG C^AATTG
NheI 59 GCTAGC G^CTAGC
PshBI 8405 ATTAAT AT^TAAT
RsrII 2254 CGGACCG CG^G[AT]CCG
SbfI 5874 CCTGCAGG CCTGCA^GG
SexAI 6115 ACCAGGT A^CC[AT]GGT
Sse8387I 5874 CCTGCAGG CCTGCA^GG
XbaI 6151 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1848 GACGTC GACGT^C
7039 GACGTC
AflII 7991 CTTAAG C^TTAAG
8537 CTTAAG
AhdI 3453 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8360 GACTAGGAGTC
AspEI 3453 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8360 GACTAGGAGTC
AspI 5039 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7981 GACTTTGTC
AvrII 7525 CCTAGG C^CTAGG
8549 CCTAGG
BfrI 7991 CTTAAG C^TTAAG
8537 CTTAAG
BlnI 7525 CCTAGG C^CTAGG
8549 CCTAGG
BlpI 7934 GCTGAGC GC^T[ACGT]AGC
8263 GCTTAGC
Bpu1102I 7934 GCTGAGC GC^T[ACGT]AGC
8263 GCTTAGC
BsaBI 507 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8341 GATTAGGATC
BsmBI 5816 CGTCTC CGTCTC(1/5)
6276 CGTCTC
BspHI 7115 TCATGA T^CATGA
8461 TCATGA
BstBI 1041 TTCGAA TT^CGAA
3005 TTCGAA
CelII 7934 GCTGAGC GC^T[ACGT]AGC
8263 GCTTAGC
DraIII 581 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
6648 CACTTAGTG
Eam1105I 3453 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8360 GACTAGGAGTC
HincII 1851 GTCAAC GT[CT]^[GA]AC
6949 GTCAAC
HindII 1851 GTCAAC GT[CT]^[GA]AC
6949 GTCAAC
MamI 507 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8341 GATTAGGATC
NspV 1041 TTCGAA TT^CGAA
3005 TTCGAA
PflFI 5039 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7981 GACTTTGTC
PshAI 966 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5810 GACAATCGTC
RcaI 7115 TCATGA T^CATGA
8461 TCATGA
SfuI 1041 TTCGAA TT^CGAA
3005 TTCGAA
SgrAI 1353 CACCGGCG C[GA]^CCGG[CT]G
2534 CGCCGGCG
Tth111I 5039 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7981 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI