##################################
# NO CUT ENZYMES (   39 enzymes) #
##################################

AclI        AgeI        Aor51HI     AscI        AviII       
BciVI       BclI        Bsp1407I    BspDI       BsrGI       
BssHII      ClaI        Eco47III    EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KspI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmeI        Psp1406I    PvuI        SacII       
SalI        SfiI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       6119  GGTACC               G^GTACC
Alw44I        654  GTGCAC               G^TGCAC
ApaLI         654  GTGCAC               G^TGCAC
AseI         8405  ATTAAT               AT^TAAT
AsnI         8405  ATTAAT               AT^TAAT
Asp718I      6119  GGTACC               G^GTACC
BsiWI         232  CGTACG               C^GTACG
BspLU11I     1822  ACATGT               A^CATGT
Bst1107I     8593  GTATAC               GTA^TAC
BstEII       4307  GGTAACC              G^GT[ACGT]ACC
BstPI        4307  GGTAACC              G^GT[ACGT]ACC
BstZ17I      8593  GTATAC               GTA^TAC
CpoI         2254  CGGACCG              CG^G[AT]CCG
EcoO65I      4307  GGTAACC              G^GT[ACGT]ACC
EcoRI        6758  GAATTC               G^AATTC
EcoRV        7132  GATATC               GAT^ATC
KpnI         6119  GGTACC               GGTAC^C
MfeI         7882  CAATTG               C^AATTG
MunI         7882  CAATTG               C^AATTG
NheI           59  GCTAGC               G^CTAGC
PshBI        8405  ATTAAT               AT^TAAT
RsrII        2254  CGGACCG              CG^G[AT]CCG
SbfI         5874  CCTGCAGG             CCTGCA^GG
SexAI        6115  ACCAGGT              A^CC[AT]GGT
Sse8387I     5874  CCTGCAGG             CCTGCA^GG
XbaI         6151  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII        1848  GACGTC               GACGT^C
             7039  GACGTC              
AflII        7991  CTTAAG               C^TTAAG
             8537  CTTAAG              
AhdI         3453  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8360  GACTAGGAGTC         
AspEI        3453  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8360  GACTAGGAGTC         
AspI         5039  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7981  GACTTTGTC           
AvrII        7525  CCTAGG               C^CTAGG
             8549  CCTAGG              
BfrI         7991  CTTAAG               C^TTAAG
             8537  CTTAAG              
BlnI         7525  CCTAGG               C^CTAGG
             8549  CCTAGG              
BlpI         7934  GCTGAGC              GC^T[ACGT]AGC
             8263  GCTTAGC             
Bpu1102I     7934  GCTGAGC              GC^T[ACGT]AGC
             8263  GCTTAGC             
BsaBI         507  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             8341  GATTAGGATC          
BsmBI        5816  CGTCTC               CGTCTC(1/5)
             6276  CGTCTC              
BspHI        7115  TCATGA               T^CATGA
             8461  TCATGA              
BstBI        1041  TTCGAA               TT^CGAA
             3005  TTCGAA              
CelII        7934  GCTGAGC              GC^T[ACGT]AGC
             8263  GCTTAGC             
DraIII        581  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6648  CACTTAGTG           
Eam1105I     3453  GACACCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8360  GACTAGGAGTC         
HincII       1851  GTCAAC               GT[CT]^[GA]AC
             6949  GTCAAC              
HindII       1851  GTCAAC               GT[CT]^[GA]AC
             6949  GTCAAC              
MamI          507  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             8341  GATTAGGATC          
NspV         1041  TTCGAA               TT^CGAA
             3005  TTCGAA              
PflFI        5039  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7981  GACTTTGTC           
PshAI         966  GACCAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5810  GACAATCGTC          
RcaI         7115  TCATGA               T^CATGA
             8461  TCATGA              
SfuI         1041  TTCGAA               TT^CGAA
             3005  TTCGAA              
SgrAI        1353  CACCGGCG             C[GA]^CCGG[CT]G
             2534  CGCCGGCG            
Tth111I      5039  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7981  GACTTTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  166 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AflIII      AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluI        
MluNI       MnlI        MroI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
XmnI