##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AgeI AscI AseI AsnI
AviII BciVI BclI BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI MamI MroI NdeI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SfiI SfuI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 6342 GGTACC G^GTACC
AcsI 7568 GAATTT [GA]^AATT[CT]
AflII 6914 CTTAAG C^TTAAG
AhdI 4038 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1248 GTGCAC G^TGCAC
ApaLI 1248 GTGCAC G^TGCAC
ApoI 7568 GAATTT [GA]^AATT[CT]
Asp718I 6342 GGTACC G^GTACC
AspEI 4038 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6593 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6224 CCTAGG C^CTAGG
BamHI 2087 GGATCC G^GATCC
BfrI 6914 CTTAAG C^TTAAG
BlnI 6224 CCTAGG C^CTAGG
Bsp1407I 7718 TGTACA T^GTACA
BsrDI 6934 CATTGC GCAATG(2/0)
BsrGI 7718 TGTACA T^GTACA
Eam1105I 4038 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 6342 GGTACC GGTAC^C
MfeI 6779 CAATTG C^AATTG
MluI 3510 ACGCGT A^CGCGT
MunI 6779 CAATTG C^AATTG
NcoI 7272 CCATGG C^CATGG
NheI 5130 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 6593 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 6924 GAAGAGC GCTCTTC(1/4)
SexAI 6611 ACCAGGT A^CC[AT]GGT
SgrAI 1687 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 7718 TGTACA T^GTACA
SspI 969 AATATT AAT^ATT
Tth111I 6593 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 612 GTCTAC GT^[AC][GT]AC
5304 GTCTAC
AclI 3719 AACGTT AA^CGTT
7097 AACGTT
Aor51HI 103 AGCGCT AGC^GCT
284 AGCGCT
BbrPI 4677 CACGTG CAC^GTG
5038 CACGTG
BcgI 2197 CGACACCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3662 CGATGAAATTGC
BglII 1198 AGATCT A^GATCT
5707 AGATCT
BsmI 1187 GAATGC GAATGC(1/-1)
6710 GAATGC
BssHII 248 GCGCGC G^CGCGC
3516 GCGCGC
DraIII 2775 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
7190 CACCCTGTG
DrdI 4919 GACAGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6137 GACCTATAAGTC
Eco47III 103 AGCGCT AGC^GCT
284 AGCGCT
PmaCI 4677 CACGTG CAC^GTG
5038 CACGTG
PmlI 4677 CACGTG CAC^GTG
5038 CACGTG
Psp1406I 3719 AACGTT AA^CGTT
7097 AACGTT
SbfI 1697 CCTGCAGG CCTGCA^GG
3701 CCTGCAGG
SphI 1141 GCATGC GCATG^C
7303 GCATGC
Sse8387I 1697 CCTGCAGG CCTGCA^GG
3701 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI
XmnI