##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BbeI        BlnI        BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoO65I     FseI        KasI        
KpnI        KspI        MroI        NarI        NdeI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

Alw44I       7314  GTGCAC               G^TGCAC
Aor51HI      3358  AGCGCT               AGC^GCT
ApaLI        7314  GTGCAC               G^TGCAC
AviII        3774  TGCGCA               TGC^GCA
BbrPI        7231  CACGTG               CAC^GTG
BglI         1287  GCCGGAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        7231  CACGTG               [CT]AC^GT[GA]
BssSI        5123  CACGAG               CACGAG(-5/-1)
Eco47III     3358  AGCGCT               AGC^GCT
EcoT22I      3494  ATGCAT               ATGCA^T
FspI         3774  TGCGCA               TGC^GCA
HpaI           67  GTTAAC               GTT^AAC
MfeI         2340  CAATTG               C^AATTG
MluI         6899  ACGCGT               A^CGCGT
MunI         2340  CAATTG               C^AATTG
NaeI         2063  GCCGGC               GCC^GGC
NgoMIV       2063  GCCGGC               G^CCGGC
NheI         5550  GCTAGC               G^CTAGC
NsiI         3494  ATGCAT               ATGCA^T
PmaCI        7231  CACGTG               CAC^GTG
PmlI         7231  CACGTG               CAC^GTG
PshAI        6716  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        6188  ACCTGGT              A^CC[AT]GGT
SmaI         4776  CCCGGG               CCC^GGG
XmaCI        4776  CCCGGG               C^CCGGG
XmaI         4776  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         713  CGCG                 CG^CG
             6900  CGCG                
AcyI         3225  GACGCC               G[GA]^CG[CT]C
             4642  GACGCC              
AflII        2872  CTTAAG               C^TTAAG
             5583  CTTAAG              
AocI         3090  CCTGAGG              CC^T[ACGT]AGG
             7248  CCTAAGG             
BanI          629  GGTGCC               G^G[CT][GA]CC
             5395  GGTGCC              
BfrI         2872  CTTAAG               C^TTAAG
             5583  CTTAAG              
BsaHI        3225  GACGCC               G[GA]^CG[CT]C
             4642  GACGCC              
BsaWI        2259  TCCGGT               [AT]^CCGG[AT]
             4456  TCCGGT              
BstUI         713  CGCG                 CG^CG
             6900  CGCG                
BstXI        5502  CCATGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6791  CCATTCCTCTGG        
Bsu36I       3090  CCTGAGG              CC^T[ACGT]AGG
             7248  CCTAAGG             
DraIII        950  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3922  CACTGTGTG           
Eco81I       3090  CCTGAGG              CC^T[ACGT]AGG
             7248  CCTAAGG             
Hin1I        3225  GACGCC               G[GA]^CG[CT]C
             4642  GACGCC              
MvnI          713  CGCG                 CG^CG
             6900  CGCG                
SacI         3050  GAGCTC               GAGCT^C
             5844  GAGCTC              
ScaI         2669  AGTACT               AGT^ACT
             5650  AGTACT              
XbaI         4120  TCTAGA               T^CTAGA
             6267  TCTAGA              
XcmI         2078  CCACAGACAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             7272  CCAGAGCAGTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRI       
EcoRII      EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI