################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BlnI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I FseI KasI KpnI KspI MroI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 7314 GTGCAC G^TGCAC Aor51HI 3358 AGCGCT AGC^GCT ApaLI 7314 GTGCAC G^TGCAC AviII 3774 TGCGCA TGC^GCA BbrPI 7231 CACGTG CAC^GTG BglI 1287 GCCGGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 7231 CACGTG [CT]AC^GT[GA] BssSI 5123 CACGAG CACGAG(-5/-1) Eco47III 3358 AGCGCT AGC^GCT EcoT22I 3494 ATGCAT ATGCA^T FspI 3774 TGCGCA TGC^GCA HpaI 67 GTTAAC GTT^AAC MfeI 2340 CAATTG C^AATTG MluI 6899 ACGCGT A^CGCGT MunI 2340 CAATTG C^AATTG NaeI 2063 GCCGGC GCC^GGC NgoMIV 2063 GCCGGC G^CCGGC NheI 5550 GCTAGC G^CTAGC NsiI 3494 ATGCAT ATGCA^T PmaCI 7231 CACGTG CAC^GTG PmlI 7231 CACGTG CAC^GTG PshAI 6716 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 6188 ACCTGGT A^CC[AT]GGT SmaI 4776 CCCGGG CCC^GGG XmaCI 4776 CCCGGG C^CCGGG XmaI 4776 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 713 CGCG CG^CG 6900 CGCG AcyI 3225 GACGCC G[GA]^CG[CT]C 4642 GACGCC AflII 2872 CTTAAG C^TTAAG 5583 CTTAAG AocI 3090 CCTGAGG CC^T[ACGT]AGG 7248 CCTAAGG BanI 629 GGTGCC G^G[CT][GA]CC 5395 GGTGCC BfrI 2872 CTTAAG C^TTAAG 5583 CTTAAG BsaHI 3225 GACGCC G[GA]^CG[CT]C 4642 GACGCC BsaWI 2259 TCCGGT [AT]^CCGG[AT] 4456 TCCGGT BstUI 713 CGCG CG^CG 6900 CGCG BstXI 5502 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6791 CCATTCCTCTGG Bsu36I 3090 CCTGAGG CC^T[ACGT]AGG 7248 CCTAAGG DraIII 950 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3922 CACTGTGTG Eco81I 3090 CCTGAGG CC^T[ACGT]AGG 7248 CCTAAGG Hin1I 3225 GACGCC G[GA]^CG[CT]C 4642 GACGCC MvnI 713 CGCG CG^CG 6900 CGCG SacI 3050 GAGCTC GAGCT^C 5844 GAGCTC ScaI 2669 AGTACT AGT^ACT 5650 AGTACT XbaI 4120 TCTAGA T^CTAGA 6267 TCTAGA XcmI 2078 CCACAGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 7272 CCAGAGCAGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI