##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I BsiWI BspHI BssHII
BstBI DraI HindIII HpaI KpnI
KspI MluI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
SacII SalI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 6805 CTTAAG C^TTAAG
AhdI 2029 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2029 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2000 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5460 CCTAGG C^CTAGG
BcgI 5325 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 11 TGATCA T^GATCA
BfrI 6805 CTTAAG C^TTAAG
BlnI 5460 CCTAGG C^CTAGG
BsaBI 6897 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5955 CGGCCG CG[GA][CT]^CG
BsmBI 897 GAGACG CGTCTC(1/5)
BsmI 3787 GCATTC GAATGC(1/-1)
BspDI 1516 ATCGAT AT^CGAT
BsrDI 7034 GCAATG GCAATG(2/0)
BssSI 2737 CACGAG CACGAG(-5/-1)
Bst1107I 2101 GTATAC GTA^TAC
BstEII 1230 GGTCACC G^GT[ACGT]ACC
BstPI 1230 GGTCACC G^GT[ACGT]ACC
BstZ17I 2101 GTATAC GTA^TAC
ClaI 1516 ATCGAT AT^CGAT
EagI 5955 CGGCCG C^GGCCG
Eam1105I 2029 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5955 CGGCCG C^GGCCG
Eco52I 5955 CGGCCG C^GGCCG
EcoO65I 1230 GGTCACC G^GT[ACGT]ACC
EcoRI 4436 GAATTC G^AATTC
EcoRV 2359 GATATC GAT^ATC
FbaI 11 TGATCA T^GATCA
FseI 508 GGCCGGCC GGCCGG^CC
MamI 6897 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 52 CAATTG C^AATTG
MunI 52 CAATTG C^AATTG
PflFI 2000 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 1320 CCTGCAGG CCTGCA^GG
SexAI 2827 ACCTGGT A^CC[AT]GGT
SfiI 4451 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1320 CCTGCAGG CCTGCA^GG
Tth111I 2000 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 3043 GACGTC GACGT^C
6813 GACGTC
AccI 2101 GTATAC GT^[AC][GT]AC
6835 GTCTAC
AcsI 2848 AAATTC [GA]^AATT[CT]
4436 GAATTC
ApoI 2848 AAATTC [GA]^AATT[CT]
4436 GAATTC
Asp700I 1470 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4134 GAAAGTCTTC
BalI 3474 TGGCCA TGG^CCA
6785 TGGCCA
BbrPI 851 CACGTG CAC^GTG
7093 CACGTG
BciVI 1821 GGATAC GTATCC(6/5)
6024 GGATAC
BglII 1079 AGATCT A^GATCT
1604 AGATCT
BspLU11I 1707 ACATGT A^CATGT
6665 ACATGT
CpoI 3270 CGGACCG CG^G[AT]CCG
3554 CGGACCG
EcoT22I 2918 ATGCAT ATGCA^T
7027 ATGCAT
MluNI 3474 TGGCCA TGG^CCA
6785 TGGCCA
MscI 3474 TGGCCA TGG^CCA
6785 TGGCCA
NaeI 509 GCCGGC GCC^GGC
2465 GCCGGC
NgoMIV 509 GCCGGC G^CCGGC
2465 GCCGGC
NsiI 2918 ATGCAT ATGCA^T
7027 ATGCAT
PmaCI 851 CACGTG CAC^GTG
7093 CACGTG
PmlI 851 CACGTG CAC^GTG
7093 CACGTG
RsrII 3270 CGGACCG CG^G[AT]CCG
3554 CGGACCG
ScaI 3641 AGTACT AGT^ACT
6908 AGTACT
XmnI 1470 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4134 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AspHI
AvaI AvaII AviII BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspEI
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI